IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/02: Difference between revisions
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* Ligated inserts into 50ng of B21 v0120 backbone with a 3:1 insert to backbone ratio | * Ligated inserts into 50ng of B21 v0120 backbone with a 3:1 insert to backbone ratio | ||
* * Transformed and plated on LB+AMP plates and left in 37°C incubator overnight | ** Transformed and plated on LB+AMP plates and left in 37°C incubator overnight | ||
===Gel Purification=== | ===Gel Purification=== |
Revision as of 08:23, 3 August 2010
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Team FenceGel extractionGal4 PCR product and Ec digest gel extract and purified. Gal on the left, EcR on the right VP16 PCR 2No PCR product is visible
Team FlavorDTL of Mira/Brazz+StrepII+Stop into V24
DTL of Wintergreen pathway
Gel Purification
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