IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/29: Difference between revisions
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===Digest of Wednesday's minipreps=== | ===Digest of Wednesday's minipreps=== | ||
====Samples 1-9==== | |||
[[Image: 729 digest 1-9.jpg|500 px]] | [[Image: 729 digest 1-9.jpg|500 px]] | ||
*Lane 1: ladder | *Lane 1: ladder | ||
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====Samples 10-18==== | ====Samples 10-18==== | ||
[[Image: 729 digest 10-18.jpg|500 px]] | |||
====Samples 19-24==== | |||
[[Image: 729 digest 19-24.jpg|500 px]] | |||
==Team Flavor== | ==Team Flavor== |
Revision as of 11:28, 29 July 2010
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Team Allergy
Gel: ladder, 9, 9, 11, 11, 25, 25, 28, 28, ladder, 36, 36, 37, 37, 38, 38, ladder, LTPS+ PDK, LTPS+PDK, Bet2S+PDK, Bet2S+PDK, GerS+PDK, GerS+PDK, GerS+PDK, GerS+PDK Expected band lengths: Complete Parts 9,11 (LTPS+Pal+LTPA): 840bp and 3200 bp 25,28 (Bet2S+Pal+Bet2A): 840bp and 3200 bp 36,37,38 (GerS+Pal+GerA): 840bp and 3200 bp Sense + Intron Parts LTPS+ PDK: 1200 and 3200 bp Bet2S+ PDK: 1200 and 3200 bp GerS+PDK: 1200 and 3200 bp (9 c1, 25 c2, 36 c1 have a 540 and 3200 band as opposed to a 840 and 3200 band--prbly b/c sense+intron is there but antisense was not ligated in). All intron+sense parts except for LTPS+PDK appear to have 300 bands as well indicating that PDK was not ligated in. (a small mix up with two of the lids was made and its possible that LTPS+PDK (w/ an 840 and 3200 band is actually the complete Bet2 part so we're sending this in for sequening anyway). Team FenceDigest of Wednesday's miniprepsSamples 1-9
insert sizes: ACC synthase: 300 to 350 Terminator: unknown Act2LacI: ~1200 Samples 10-18Samples 19-24Team FlavorMinipreps
Gel Extraction
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