Difference between revisions of "IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/28"

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(Team Flavor)
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* Ran gel to confirm Valencene PCR: <font color="red">failed</font>
 
* Ran gel to confirm Valencene PCR: <font color="red">failed</font>
 
[[Image:ValPfxConfirm.jpg|240px]]
 
[[Image:ValPfxConfirm.jpg|240px]]
 +
# Ladder
 +
# DNA 1-1
 +
# DNA 1-2
 +
# DNA 2-1
 +
# DNA 2-2
 +
''The numerical differentiation refers to the specific genomic DNA sample''
  
 
* Ran PCR to extract J45004 and J45017 parts from the Wintergreen Pathway
 
* Ran PCR to extract J45004 and J45017 parts from the Wintergreen Pathway

Revision as of 11:36, 28 July 2010

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Team Flavor

  • Ran gel to confirm Valencene PCR: failed

ValPfxConfirm.jpg

  1. Ladder
  2. DNA 1-1
  3. DNA 1-2
  4. DNA 2-1
  5. DNA 2-2

The numerical differentiation refers to the specific genomic DNA sample

  • Ran PCR to extract J45004 and J45017 parts from the Wintergreen Pathway
   Primers: 
    J45004_F
    Left Primer: 5' cctttctagaatggaagttgttgaagttcttca 3'
    J45004_R
    Right Primer: 5' aaggctgcagcggccgctactagtttaatttattttggtcaagga 3' (last 5 bp omitted to meet 45 bp maximum)
    J45017_F
    Left Primer: 5' cctttctagaatgaaaactcccgaagactgc 3'
    J45017_R
    Right Primer: 5' aaggctgcagcggccgctactagtttattaggcgacgccgc 3'