Difference between revisions of "IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/06/18"

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(Team Vector)
(Team Vector)
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<td>nptll</td>
 
<td>nptll</td>
 
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<p>To create the vector backbones we digested the E and R plasmids with HindIII and SpeI, and the O plasmids with SacII and SpeI. We ran the digested backbones on a gel and purified using the Qiaquick Gel Extraction kit. IMAGE HERE</p>
 
<p>To create the vector backbones we digested the E and R plasmids with HindIII and SpeI, and the O plasmids with SacII and SpeI. We ran the digested backbones on a gel and purified using the Qiaquick Gel Extraction kit. IMAGE HERE</p>
  

Revision as of 12:25, 18 June 2010

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Week 1 Summary (6/18/2010)

Team Vector

File:PORE Vectors.xls

<html> Aim: To biobrick agrobacteria vectors<br /><br />

We obtained the following pORE agrobacteria vectors from TAIR.<br /><br /> <table> <tr> <td>Name</td> <td>Original Name</td> <td>Description</td> <td>Plant Resistance</td> </tr> <tr> <td>V1</td> <td>pORE_O1</td> <td>Agrobacterium vector open series</td> <td>pat</td> </tr> <tr> <td>V2</td> <td>pORE_O2</td> <td>Agrobacterium vector open series</td> <td>nptll</td> </tr> <tr> <td>V3</td> <td>pORE_E3</td> <td>Agrobacterium vector with ENTCUP2 promoter</td> <td>pat</td> </tr> <tr> <td>V4</td> <td>pORE_E4</td> <td>Agrobacterium vector with ENTCUP2 promoter</td> <td>nptll</td> </tr> <tr> <td>V5</td> <td>pORE_R1</td> <td>Agrobacterium vector with gusA reporter</td> <td>nptll</td> </tr> <tr> <td>V6</td> <td>pORE_R3</td> <td>Agrobacterium vector with smgfp reporter</td> <td>nptll</td> </tr> </table><br /> <p>To create the vector backbones we digested the E and R plasmids with HindIII and SpeI, and the O plasmids with SacII and SpeI. We ran the digested backbones on a gel and purified using the Qiaquick Gel Extraction kit. IMAGE HERE</p>

</html>

Team Flavor

Team Allergy

Progress

  1. Identified allergens in arabidopsis, strawberries,tomatoes, and carrots and created primers for amplifying out these allergens (see primers and sequences)
  2. Extracted cDNA (rna extraction followed by rt-pcr) from strawberries and tried to PCR out our strawberry allergen (Fra a 1), but were unble ato do so
  3. Looked to find introns ~ 800 bp to use to create our hairpins since it was difficult to acquire pHannibal vector w/ the pdk intron

Future Directions

  1. Look towards ordering amiRNAfor silencing
  2. Waiting on arabidopsis plants to grow to extract genomic DNA (mon)
  3. Look to find introns to use in creation of hairpin DNA
  4. Cloning (digesting/ligating our sense/antisense/intron sequences necessary for hairpin creation into one vector to create biobricks)

Team Fence