Difference between revisions of "IGEM:Harvard/2008/Plasmids"

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(Parts)
(Parts)
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| P38 || [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23114 BBa_J23114]||High constitutive promoter||||||Amp||||
 
| P38 || [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23114 BBa_J23114]||High constitutive promoter||||||Amp||||
 
|-
 
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| P39 || [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23113 BBa-J23113]||Low constitutive promoter||||||Amp||||
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| P39 || [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23113 BBa_J23113]||Low constitutive promoter||||||Amp||||
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|-
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| P40 || [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0032 BBa_B0032]||Ribosome Binding Site||||||Amp||||
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|-
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| P41 || [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0010 BBa_B0010]||Transcriptional Terminator||||||Amp||||
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| P42 || [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0012 BBa_C0012]||LacI coding region||||||Amp||||
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| P43 || [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0040]||TetR coding region||||||Amp||||
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| P44 || [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0051 BBa_C0051]||cI lambda||||||Amp||||
 
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Revision as of 11:36, 27 June 2008

Vectors

Parts

Name Registry Name Description Origin Size Marker Marker Miniprepped? Culture in Glycerol Stock?
P1 J23113 Low promoter GFP tester pMB1 35 Kan Yes in S1
P2 J23150/I20269 Medium promoter GFP tester p15A pMR101-derived 919 Kan
P3 J23151/I20270 High promoter GFP tester p15A pMR101-derived 919 Kan Yes in S1
P4 pACYC duet Low-Medium copy vector p15a 4008 Cm Yes in S1 and TOP10
P5 pSB3K3 Low-Medium copy vector (w/ death gene) p15a 2750 Kan
P6 BBa-E1010 RFP only (w/ death gene) pMB1 681 Kan
P7 pSB1A2 GFP only pMB1 2079 Amp
P8 BBa_J04450 RFP with LacI promoter pMB1 1069 Amp
P9 BBa_J04430 GFP with LacI promoter pMB1 1083 Amp
P10 BBa_I715038 T7 Polymerase with LacI promoter pMB1 2878 Amp
P11 BBa_M30109 Light responsive system, dual regulation pUC19-derived pMB1 4333 Amp/Cm
P12 pETDuet-1 Low copy plasmid pBR322-derived ColE1 5420 Amp
P13 pCDFDuet-1 Low copy plasmid CloDF13 3781 Sm Yes in S1
P14 pCOLADuet-1 Low copy plasmid ColA 3719 Kan
P15 BBa_I13522 GFP with Tet promoter ColE1 937 Amp
P16 BBa_R0040 Tet repressible promoter pMB1 54 Amp
P17 BBa_Q04400 Inverter (TetR w/o promoter and Tet promoter) OriS, P1 lytic, F1 902 Kan
P18 BBa_R0051 lambda promoter (cI regulated) pMB1 49 Amp
P19 BBa_I763007 RFP with lambda promoter pMB1 918 Amp
P20 BBa_J04431 GFP (LVA tagged) with Lac promoter pMB1 1122 Amp, Kan
P21 A1 (pACYC) pACYC (P4) w/ biobricks ??? ??? ??? Yes in S1 and TOP10
P22 BBa_R0082 ompR controlled promoter for ompC (long) pMB1 108 Amp
P23 BBa_R0083 ompR controlled promoter for ompC (short) pMB1 78 Amp
P24 BBa_R0084 ompR controlled promoter for ompF pMB1 108 Amp
P25 BBa_J58102 Promoter activated by OmpR-P with the reporter GFP pUC19-derived pMB1 991 Amp
P26 BBa_J58103 Mutated promoter activated by OmpR-P with the reporter GFP pUC19-derived pMB1 961 Amp
P27 pZS4int2 repressor plasmid pSC101 Sm Yes
P28 pZE21 GFP ColE1 Kan Yes
P29 pZS*2R Venus (mutant YFP) pSC101* Kan Yes
P30 pZA32 YFP p15A Cm Yes
P31 pZS*1R Venus pSC101* Amp Yes
P32 pZA32 Venus p15A Cm Yes
P33 pZA31 Venus p15A Cm Yes
P34 pZE12 GFP ColE1 Amp Yes
P35 pLDR8 Yes
P36 BBa_I763004 GFP + IPTG + LVA Kan/Amp
P37 BBa_I722007 Constructive expression LacI w/ pTetR promoter Amp
P38 BBa_J23114 High constitutive promoter Amp
P39 BBa_J23113 Low constitutive promoter Amp
P40 BBa_B0032 Ribosome Binding Site Amp
P41 BBa_B0010 Transcriptional Terminator Amp
P42 BBa_C0012 LacI coding region Amp
P43 [1] TetR coding region Amp
P44 BBa_C0051 cI lambda Amp