Difference between revisions of "IGEM:Harvard/2008/Plasmids"

From OpenWetWare
Jump to: navigation, search
Line 91: Line 91:
| P38 || [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23114 BBa_J23114]||High constitutive promoter||||||Amp||||
| P38 || [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23114 BBa_J23114]||High constitutive promoter||||||Amp||||
| P39 || [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23113 BBa-J23113]||Low constitutive promoter||||||Amp||||
| P39 || [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23113 BBa_J23113]||Low constitutive promoter||||||Amp||||
| P40 || [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0032 BBa_B0032]||Ribosome Binding Site||||||Amp||||
| P41 || [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0010 BBa_B0010]||Transcriptional Terminator||||||Amp||||
| P42 || [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0012 BBa_C0012]||LacI coding region||||||Amp||||
| P43 || [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0040]||TetR coding region||||||Amp||||
| P44 || [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0051 BBa_C0051]||cI lambda||||||Amp||||

Revision as of 11:36, 27 June 2008



Name Registry Name Description Origin Size Marker Marker Miniprepped? Culture in Glycerol Stock?
P1 J23113 Low promoter GFP tester pMB1 35 Kan Yes in S1
P2 J23150/I20269 Medium promoter GFP tester p15A pMR101-derived 919 Kan
P3 J23151/I20270 High promoter GFP tester p15A pMR101-derived 919 Kan Yes in S1
P4 pACYC duet Low-Medium copy vector p15a 4008 Cm Yes in S1 and TOP10
P5 pSB3K3 Low-Medium copy vector (w/ death gene) p15a 2750 Kan
P6 BBa-E1010 RFP only (w/ death gene) pMB1 681 Kan
P7 pSB1A2 GFP only pMB1 2079 Amp
P8 BBa_J04450 RFP with LacI promoter pMB1 1069 Amp
P9 BBa_J04430 GFP with LacI promoter pMB1 1083 Amp
P10 BBa_I715038 T7 Polymerase with LacI promoter pMB1 2878 Amp
P11 BBa_M30109 Light responsive system, dual regulation pUC19-derived pMB1 4333 Amp/Cm
P12 pETDuet-1 Low copy plasmid pBR322-derived ColE1 5420 Amp
P13 pCDFDuet-1 Low copy plasmid CloDF13 3781 Sm Yes in S1
P14 pCOLADuet-1 Low copy plasmid ColA 3719 Kan
P15 BBa_I13522 GFP with Tet promoter ColE1 937 Amp
P16 BBa_R0040 Tet repressible promoter pMB1 54 Amp
P17 BBa_Q04400 Inverter (TetR w/o promoter and Tet promoter) OriS, P1 lytic, F1 902 Kan
P18 BBa_R0051 lambda promoter (cI regulated) pMB1 49 Amp
P19 BBa_I763007 RFP with lambda promoter pMB1 918 Amp
P20 BBa_J04431 GFP (LVA tagged) with Lac promoter pMB1 1122 Amp, Kan
P21 A1 (pACYC) pACYC (P4) w/ biobricks ??? ??? ??? Yes in S1 and TOP10
P22 BBa_R0082 ompR controlled promoter for ompC (long) pMB1 108 Amp
P23 BBa_R0083 ompR controlled promoter for ompC (short) pMB1 78 Amp
P24 BBa_R0084 ompR controlled promoter for ompF pMB1 108 Amp
P25 BBa_J58102 Promoter activated by OmpR-P with the reporter GFP pUC19-derived pMB1 991 Amp
P26 BBa_J58103 Mutated promoter activated by OmpR-P with the reporter GFP pUC19-derived pMB1 961 Amp
P27 pZS4int2 repressor plasmid pSC101 Sm Yes
P28 pZE21 GFP ColE1 Kan Yes
P29 pZS*2R Venus (mutant YFP) pSC101* Kan Yes
P30 pZA32 YFP p15A Cm Yes
P31 pZS*1R Venus pSC101* Amp Yes
P32 pZA32 Venus p15A Cm Yes
P33 pZA31 Venus p15A Cm Yes
P34 pZE12 GFP ColE1 Amp Yes
P35 pLDR8 Yes
P36 BBa_I763004 GFP + IPTG + LVA Kan/Amp
P37 BBa_I722007 Constructive expression LacI w/ pTetR promoter Amp
P38 BBa_J23114 High constitutive promoter Amp
P39 BBa_J23113 Low constitutive promoter Amp
P40 BBa_B0032 Ribosome Binding Site Amp
P41 BBa_B0010 Transcriptional Terminator Amp
P42 BBa_C0012 LacI coding region Amp
P43 [1] TetR coding region Amp
P44 BBa_C0051 cI lambda Amp