IGEM:Harvard/2007/Plates

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Colony Count of Overnight Ligation vs. Shorter (2 hr Ligation) Plated 6-20-07

Cell Type Volume Plated Colony Count
(Short Ligation)
Colony Count
(Overnight Ligation)
Colony Count
(Overweekend Ligation;1uL DNA)
Colony Count
(Overweekend Ligation;6uL DNA)
OmpA1 + random library, PCR 275 µL 5 41 10 119
OmpA1 + random library, PCR 25 µL 0 2 n/a n/a
OmpA1 + random library, extension 275 µL 0 30 0 127
OmpA1 + random library, extension 25 µL 0 0 n/a n/a
OmpA2 + random library, PCR 275 µL 0 1
OmpA2 + random library, PCR 25 µL 0 0 n/a n/a
OmpA2 + random library, extension 275 µL 12 77
OmpA2 + random library, extension 25 µL 0 1 n/a n/a