IGEM:Harvard/2007/Plates
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Colony Count of Overnight Ligation vs. Shorter (2 hr Ligation) Plated 6-20-07
Cell Type | Volume Plated | Colony Count (Short Ligation) |
Colony Count (Overnight Ligation) |
OmpA1 + random library, PCR | 275 µL | 5 | 41 |
OmpA1 + random library, PCR | 25 µL | 0 | 2 |
OmpA1 + random library, extension | 275 µL | 0 | 30 |
OmpA1 + random library, extension | 25 µL | 0 | 0 |
OmpA2 + random library, PCR | 275 µL | 0 | 1 |
OmpA2 + random library, PCR | 25 µL | 0 | 0 |
OmpA2 + random library, extension | 275 µL | 12 | 77 |
OmpA2 + random library, extension | 25 µL | 0 | 1 |