Difference between revisions of "IGEM:Harvard/2007/Plates"

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(Colony Count of Electroporation)
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| align="center" style="background:#f0f0f0;"|'''0ng DNA'''
 
| align="center" style="background:#f0f0f0;"|'''0ng DNA'''
 
| align="center" style="background:#f0f0f0;"|'''25ng DNA'''
 
| align="center" style="background:#f0f0f0;"|'''25ng DNA'''
| align="center" style="background:#f0f0f0;"|'''50ng DNA'''
+
| align="center" style="background:#ff0000;"|'''50ng DNA'''
 
| align="center" style="background:#f0f0f0;"|'''100ng DNA'''
 
| align="center" style="background:#f0f0f0;"|'''100ng DNA'''
 
| align="center" style="background:#f0f0f0;"|'''250ng DNA'''
 
| align="center" style="background:#f0f0f0;"|'''250ng DNA'''

Revision as of 09:06, 12 July 2007

Colony Count of Overnight Ligation vs. Shorter (2 hr Ligation)

Plated 6-20-07

Cell Type Volume Plated Colony Count
(Short Ligation)
Colony Count
(Overnight Ligation)
OmpA1 + random library, PCR 275 µL 5 41
OmpA1 + random library, PCR 25 µL 0 2
OmpA1 + random library, extension 275 µL 0 30
OmpA1 + random library, extension 25 µL 0 0
OmpA2 + random library, PCR 275 µL 0 1
OmpA2 + random library, PCR 25 µL 0 0
OmpA2 + random library, extension 275 µL 12 77
OmpA2 + random library, extension 25 µL 0 1

Colony Count of Overweekend Ligation

Plated 6/25/07 The following were all done with 275 uL:

Cell Type Colony Count
(Overweekend Ligation;1uL DNA)
Colony Count
(Overweekend Ligation;6uL DNA)
OmpA1/Group1/S 0 127
OmpA1/Group1/S2 8 24
OmpA1/Group2/B 10 119
OmpA1/Group2/S2 4 79

Colony Count of Electroporation

Plated 7/11/07

Plate Dilution of cells 0ng DNA 25ng DNA 50ng DNA 100ng DNA 250ng DNA 500ng DNA
LB-KAN 10^-2 521 136 197 361
LB 10^-3 489 329 576 267 130 338