Difference between revisions of "IGEM:Harvard/2007/Plates"

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| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Overweekend Ligation;6uL DNA)'''
 
| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Overweekend Ligation;6uL DNA)'''
 
|-
 
|-
| OmpA1 + random library, PCR||275 µL||5||41
+
| OmpA1 + random library, PCR||275 µL||5||41||10||119
 
|-
 
|-
 
| OmpA1 + random library, PCR||25 µL||0||2||n/a||n/a
 
| OmpA1 + random library, PCR||25 µL||0||2||n/a||n/a
 
|-
 
|-
| OmpA1 + random library, extension||275 µL||0||30
+
| OmpA1 + random library, extension||275 µL||0||30||0||127
 
|-
 
|-
 
| OmpA1 + random library, extension||25 µL||0||0||n/a||n/a
 
| OmpA1 + random library, extension||25 µL||0||0||n/a||n/a

Revision as of 08:20, 26 June 2007

Colony Count of Overnight Ligation vs. Shorter (2 hr Ligation) Plated 6-20-07

Cell Type Volume Plated Colony Count
(Short Ligation)
Colony Count
(Overnight Ligation)
Colony Count
(Overweekend Ligation;1uL DNA)
Colony Count
(Overweekend Ligation;6uL DNA)
OmpA1 + random library, PCR 275 µL 5 41 10 119
OmpA1 + random library, PCR 25 µL 0 2 n/a n/a
OmpA1 + random library, extension 275 µL 0 30 0 127
OmpA1 + random library, extension 25 µL 0 0 n/a n/a
OmpA2 + random library, PCR 275 µL 0 1
OmpA2 + random library, PCR 25 µL 0 0 n/a n/a
OmpA2 + random library, extension 275 µL 12 77
OmpA2 + random library, extension 25 µL 0 1 n/a n/a