Difference between revisions of "IGEM:Harvard/2007/Plates"

From OpenWetWare
Jump to: navigation, search
Line 11: Line 11:
 
| OmpA1 + random library, PCR||275 µL||5||41
 
| OmpA1 + random library, PCR||275 µL||5||41
 
|-
 
|-
| OmpA1 + random library, PCR||25 µL||0||2
+
| OmpA1 + random library, PCR||25 µL||0||2||n/a||n/a
 
|-
 
|-
 
| OmpA1 + random library, extension||275 µL||0||30
 
| OmpA1 + random library, extension||275 µL||0||30
 
|-
 
|-
| OmpA1 + random library, extension||25 µL||0||0
+
| OmpA1 + random library, extension||25 µL||0||0||n/a||n/a
 
|-
 
|-
 
| OmpA2 + random library, PCR||275 µL||0||1
 
| OmpA2 + random library, PCR||275 µL||0||1
 
|-
 
|-
| OmpA2 + random library, PCR||25 µL||0||0
+
| OmpA2 + random library, PCR||25 µL||0||0||n/a||n/a
 
|-
 
|-
 
| OmpA2 + random library, extension||275 µL||12||77
 
| OmpA2 + random library, extension||275 µL||12||77
 
|-
 
|-
| OmpA2 + random library, extension||25 µL||0||1
+
| OmpA2 + random library, extension||25 µL||0||1||n/a||n/a
 
|}
 
|}

Revision as of 09:17, 26 June 2007

Colony Count of Overnight Ligation vs. Shorter (2 hr Ligation) Plated 6-20-07

Cell Type Volume Plated Colony Count
(Short Ligation)
Colony Count
(Overnight Ligation)
Colony Count
(Overweekend Ligation;1uL DNA)
Colony Count
(Overweekend Ligation;6uL DNA)
OmpA1 + random library, PCR 275 µL 5 41
OmpA1 + random library, PCR 25 µL 0 2 n/a n/a
OmpA1 + random library, extension 275 µL 0 30
OmpA1 + random library, extension 25 µL 0 0 n/a n/a
OmpA2 + random library, PCR 275 µL 0 1
OmpA2 + random library, PCR 25 µL 0 0 n/a n/a
OmpA2 + random library, extension 275 µL 12 77
OmpA2 + random library, extension 25 µL 0 1 n/a n/a