Difference between revisions of "IGEM:Harvard/2007/Plates"

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| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Short Ligation)'''
 
| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Short Ligation)'''
 
| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Overnight Ligation)'''
 
| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Overnight Ligation)'''
 +
| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Overweekend Ligation;1uL DNA)'''
 +
| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Overweekend Ligation;6uL DNA)'''
 
|-
 
|-
 
| OmpA1 + random library, PCR||275 µL||5||41
 
| OmpA1 + random library, PCR||275 µL||5||41

Revision as of 08:16, 26 June 2007

Colony Count of Overnight Ligation vs. Shorter (2 hr Ligation) Plated 6-20-07

Cell Type Volume Plated Colony Count
(Short Ligation)
Colony Count
(Overnight Ligation)
Colony Count
(Overweekend Ligation;1uL DNA)
Colony Count
(Overweekend Ligation;6uL DNA)
OmpA1 + random library, PCR 275 µL 5 41
OmpA1 + random library, PCR 25 µL 0 2
OmpA1 + random library, extension 275 µL 0 30
OmpA1 + random library, extension 25 µL 0 0
OmpA2 + random library, PCR 275 µL 0 1
OmpA2 + random library, PCR 25 µL 0 0
OmpA2 + random library, extension 275 µL 12 77
OmpA2 + random library, extension 25 µL 0 1