Difference between revisions of "IGEM:Harvard/2007/Plates"

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(New page: Colony Count of Overnight Ligation vs. Shorter (2 hr Ligation) Plated 6-20-07 {| {{table}} | align="center" style="background:#f0f0f0;"|'''Cell Type''' | align="center" style="background:#...)
 
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| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Overnight Ligation)'''
 
| align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Overnight Ligation)'''
 
|-
 
|-
| OmpA1 + random library, PCR||275 µL||41||5
+
| OmpA1 + random library, PCR||275 µL||5||41
 
|-
 
|-
| OmpA1 + random library, PCR||25 µL||2||0
+
| OmpA1 + random library, PCR||25 µL||0||2
 
|-
 
|-
| OmpA1 + random library, extension||275 µL||30||0
+
| OmpA1 + random library, extension||275 µL||0||30
 
|-
 
|-
 
| OmpA1 + random library, extension||25 µL||0||0
 
| OmpA1 + random library, extension||25 µL||0||0
 
|-
 
|-
| OmpA2 + random library, PCR||275 µL||1||0
+
| OmpA2 + random library, PCR||275 µL||0||1
 
|-
 
|-
 
| OmpA2 + random library, PCR||25 µL||0||0
 
| OmpA2 + random library, PCR||25 µL||0||0
 
|-
 
|-
| OmpA2 + random library, extension||275 µL||77||12
+
| OmpA2 + random library, extension||275 µL||12||77
 
|-
 
|-
| OmpA2 + random library, extension||25 µL||1||0
+
| OmpA2 + random library, extension||25 µL||0||1
 
|}
 
|}

Revision as of 11:25, 22 June 2007

Colony Count of Overnight Ligation vs. Shorter (2 hr Ligation) Plated 6-20-07

Cell Type Volume Plated Colony Count
(Short Ligation)
Colony Count
(Overnight Ligation)
OmpA1 + random library, PCR 275 µL 5 41
OmpA1 + random library, PCR 25 µL 0 2
OmpA1 + random library, extension 275 µL 0 30
OmpA1 + random library, extension 25 µL 0 0
OmpA2 + random library, PCR 275 µL 0 1
OmpA2 + random library, PCR 25 µL 0 0
OmpA2 + random library, extension 275 µL 12 77
OmpA2 + random library, extension 25 µL 0 1