IGEM:Harvard/2007/Plates: Difference between revisions

Revision as of 11:25, 22 June 2007

Colony Count of Overnight Ligation vs. Shorter (2 hr Ligation) Plated 6-20-07

Cell Type	Volume Plated	Colony Count (Short Ligation)	Colony Count (Overnight Ligation)
OmpA1 + random library, PCR	275 µL	5	41
OmpA1 + random library, PCR	25 µL	0	2
OmpA1 + random library, extension	275 µL	0	30
OmpA1 + random library, extension	25 µL	0	0
OmpA2 + random library, PCR	275 µL	0	1
OmpA2 + random library, PCR	25 µL	0	0
OmpA2 + random library, extension	275 µL	12	77
OmpA2 + random library, extension	25 µL	0	1

@@ Line 7: / Line 7: @@
 | align="center" style="background:#f0f0f0;"|'''Colony Count <br>(Overnight Ligation)'''
 |-
-| OmpA1 + random library, PCR||275 µL||41||5
+| OmpA1 + random library, PCR||275 µL||5||41
 |-
-| OmpA1 + random library, PCR||25 µL||2||0
+| OmpA1 + random library, PCR||25 µL||0||2
 |-
-| OmpA1 + random library, extension||275 µL||30||0
+| OmpA1 + random library, extension||275 µL||0||30
 |-
 | OmpA1 + random library, extension||25 µL||0||0
 |-
-| OmpA2 + random library, PCR||275 µL||1||0
+| OmpA2 + random library, PCR||275 µL||0||1
 |-
 | OmpA2 + random library, PCR||25 µL||0||0
 |-
-| OmpA2 + random library, extension||275 µL||77||12
+| OmpA2 + random library, extension||275 µL||12||77
 |-
-| OmpA2 + random library, extension||25 µL||1||0
+| OmpA2 + random library, extension||25 µL||0||1
 |}