Difference between revisions of "IGEM:Harvard/2006/Lab Intro"

From OpenWetWare
 
m
Line 1: Line 1:
 
 
Parts:
 
Parts:
  
Line 14: Line 13:
 
* amp resistance
 
* amp resistance
  
Digest R0010 - SpeI + PstI (prefix)
+
*Digest R0010 - SpeI + PstI (prefix)
Digest E0241 - XbaI + PstI (Suffix)
+
*Digest E0241 - XbaI + PstI (Suffix)
  
 
Gel extraction -> Ligation -> Transformation -> Miniprep -> Sequencing
 
Gel extraction -> Ligation -> Transformation -> Miniprep -> Sequencing

Revision as of 10:50, 6 June 2006

Parts:

R0010 - LacI promoter

  • Plate 1, Well 7K
  • amp resistance

E0241 - GFP reporter

  • Plate 2, Well 15L
  • amp resistance

E7104 - GFP reporter behind T7 promoter

  • Plate 2, Well 13F
  • amp resistance
  • Digest R0010 - SpeI + PstI (prefix)
  • Digest E0241 - XbaI + PstI (Suffix)

Gel extraction -> Ligation -> Transformation -> Miniprep -> Sequencing