IGEM:Harvard/2006/Folding buffers

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  • for a desired final MgCl2 concentration, mix the reagents in a given row
  • yields 400 μL of 10x buffer
Final [MgCl2] in 10x Final [MgCl2] in 1x HEPES (1 M, pH 7.5) NaCl (5 M) MgCl2 (1 M) H2O
100 10 200 μL 40 μL 40 μL 120 μL
200 20 200 μL 40 μL 80 μL 80 μL
300 30 200 μL 40 μL 120 μL 40 μL

For 0.1% SDS (in 1x solution), add 20 μL 20% SDS. For 0.01% SDS (in 1x solution), add 2 μL 20% SDS.