IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-8-30
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Streptavidin Magnetic Bead Depletion Assay
- Biotinylated oligos still haven't come in yet, but the necessary accompanying "split" oligos (ie. the c5.0.#rb oligos) without biotinylation did come in.
- Reconstituted them to 200uM and put in c5.0 rack.
| oligo name | nmoles in tube | EB necessary for 200uM (uL) |
| c5.0.81rb | 44.98 | 224.9 |
| c5.0.8.2rb | 53.16 | 265.8 |
| c5.0.8.5rb | 44.72 | 223.6 |
| c5.0.9.1rb | 45.62 | 228.1 |
| c5.0.9.3rb | 40.36 | 201.8 |
| c5.0.9.5rb | 36.33 | 181.65 |
- (Previous biotinylated oligo tube orders from Invitrogen have been at ~200nmole amounts, but that has been greatly in excess of what has been actually needed and used. Thus this order was made with ~50nmole amounts, which will explain the relatively smaller amount of liquid in each tube, in case anyone is concerned about this at a later date.)
