Difference between revisions of "IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-8-17"

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Revision as of 08:17, 17 August 2006

2nd PEG Precipitation

  • conduct 2nd PEG precipitation on yesterday's precipitated samples (5.0A, C, D 7%-10%)
  • protocol: mix all ingredients, add nanostructures last, total volume is 100 mL, incubate on ice for 15 min, spin for 10 min at 16k rcf, pipet off supernatant, resuspend pellet in 100 μL water
Final PEG % PEG μL NaCl μL c5.0 μL dH2O μL
7 35 10 40 15
8 40 10 40 10
9 45 10 40 5
10 50 10 40 0


20060816 PEG.jpg
lane contents loading dye
1 8 μL untreated 5.0.A 3 μL
2 20 μL 5.0.A in 7% PEG and 0.5 M NaCl (supernatant) 3 μL
3 8 μL 5.0.A in 7% PEG and 0.5 M NaCl (pellet) 3 μL
4 20 μL 5.0.A in 8% PEG and 0.5 M NaCl (supernatant) 3 μL
5 8 μL 5.0.A in 8% PEG and 0.5 M NaCl (pellet) 3 μL
6 20 μL 5.0.A in 9% PEG and 0.5 M NaCl (supernatant) 3 μL
7 8 μL 5.0.A in 9% PEG and 0.5 M NaCl (pellet) 3 μL
8 20 μL 5.0.A in 10% PEG and 0.5 M NaCl (supernatant) 3 μL
9 8 μL 5.0.A in 10% PEG and 0.5 M NaCl (pellet) 3 μL
10 1 kb+ ladder 3 μL
11 2.7 μL p7308 3 μL
12 8 μL untreated 5.0.C 3 μL
13 20 μL 5.0.C in 7% PEG and 0.5 M NaCl (supernatant) 3 μL
14 8 μL 5.0.C in 7% PEG and 0.5 M NaCl (pellet) 3 μL
15 20 μL 5.0.C in 8% PEG and 0.5 M NaCl (supernatant) 3 μL
16 8 μL 5.0.C in 8% PEG and 0.5 M NaCl (pellet) 3 μL
17 20 μL 5.0.C in 9% PEG and 0.5 M NaCl (supernatant) 3 μL
18 8 μL 5.0.C in 9% PEG and 0.5 M NaCl (pellet) 3 μL
19 20 μL 5.0.C in 10% PEG and 0.5 M NaCl (supernatant) 3 μL
20 8 μL 5.0.C in 10% PEG and 0.5 M NaCl (pellet) 3 μL
21 8 μL untreated 5.0.D 3 μL
22 20 μL 5.0.D in 7% PEG and 0.5 M NaCl (supernatant) 3 μL
23 8 μL 5.0.D in 7% PEG and 0.5 M NaCl (pellet) 3 μL
24 20 μL 5.0.D in 8% PEG and 0.5 M NaCl (supernatant) 3 μL
25 8 μL 5.0.D in 8% PEG and 0.5 M NaCl (pellet) 3 μL
26 20 μL 5.0.D in 9% PEG and 0.5 M NaCl (supernatant) 3 μL
27 8 μL 5.0.D in 9% PEG and 0.5 M NaCl (pellet) 3 μL
28 20 μL 5.0.D in 10% PEG and 0.5 M NaCl (supernatant) 3 μL
29 8 μL 5.0.D in 10% PEG and 0.5 M NaCl (pellet) 3 μL
30 1 kb+ ladder 3 μL
31 2.7 μL p7308 3 μL
32-40 (empty) 3 μL