Difference between revisions of "IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-11-20"

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(Gel Analysis (repeat))
(Gel Analysis (repeat))
 
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[[Image:11-20foldinggel.pic]]
 
[[Image:11-20foldinggel.pic]]
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- results: the folding did not succeed, as can be seen by the lack of bands in the 3rd through 5th lanes near the position of the scaffold
 +
  band

Latest revision as of 11:45, 27 November 2006

Gel Analysis (repeat)

- samples: c5.0A (no lids, no aptamers), c5.0C (no lids, inside aptamers), c5.0D (no lids, outside aptamers)

- 2.0% agarose gel: 2 g agarose + 100 μL 0.5x TBE + 1.1 mL 1 M MgCl2 + 5 μL EtBr

- lanes:

            1 = ladder (10 μL)
            2 = p7308 scaffold (5 μL + 3 μL dye)
            3 = c5.0A (5 μL + 3 μL dye)
            4 = c5.0C (5 μL + 3 μL dye)
            5 = c5.0D (5 μL + 3 μL dye)

- run 1 hr. @ 130 V

11-20foldinggel.pic


- results: the folding did not succeed, as can be seen by the lack of bands in the 3rd through 5th lanes near the position of the scaffold

  band