Difference between revisions of "IGEM:Harvard/2006/DNA nanostructures/Notebook"

From OpenWetWare
Jump to: navigation, search
Line 18: Line 18:
==Thrombin-aptamer experiments==
====Questions / procedures====
* what percent gel? 10% to 20% polyacrylamide gels, no SDS (but would make for a good control)
* what incubation conditions?
* how much protein and DNA? protein at 1 {{um}}, DNA at 2 {{um}}
* Coomassie stain
{| {{table}}
| align="center" style="background:#f0f0f0;"|number
| align="center" style="background:#f0f0f0;"|thrombin
| align="center" style="background:#f0f0f0;"|aptamer
| align="center" style="background:#f0f0f0;"|nanotube
| align="center" style="background:#f0f0f0;"|DNA-stained prediction
| align="center" style="background:#f0f0f0;"|protein-stained prediction
|0||-||-||-||no bands||no bands
|1||-||-||+||slow band (nanotube)||no bands
|2||-||+||-||fast band (aptamer)||no bands
|3||-||+||+||slow band (aptamer-nanotube), traces of fast band (aptamer)||no bands
|4||+||-||-||no bands||fast band (thrombin)
|5||+||-||+||slow band (nanotube)||fast band (thrombin)
|6||+||+||-||medium band (aptamer-thrombin), fast band (aptamer)||medium band (aptamer-thrombin), traces of fast band (thrombin)
|7||+||+||+||very slow band (thrombin-aptamer-nanotube), slow band (aptamer-nantotube), traces of fast band (aptamer)||very slow band (thrombin-aptamer-nanotube), medium band (aptamer-thrombin), traces of fast band (thrombin)
* Macaya's and Bock's selection buffer: 20 mM Tris-acetate, pH 7.4, 140 mM NaCl, 5 mM KCl, 1 mM CaCl<sub>2</sub>, 1 mM MgCl<sub>2</sub>
* Liu's incubation buffer: 40 mM Tris, 20 mM CH<sub>3</sub>COOH, 2mM EDTA, 12.5 mM (CH<sub>3</sub>COO)<sub>2</sub>Mg, pH 8.0
* Liu's PAGE buffer: 1x TAE/Mg<sup>2+</sup>

Revision as of 12:47, 11 July 2006

<calendar> name=IGEM:Harvard/2006/DNA_nanostructures/Notebook date=2006/07/01 view=threemonths format=%name/%year-%month-%day weekstart=14 </calendar>