Difference between revisions of "IGEM:Harvard/2006/Cyanobacteria"

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(Presentations)
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*[[Media:Cyanobacteria_presentation_Week_8.ppt |Week 8 progress update]]
 
*[[Media:Cyanobacteria_presentation_Week_8.ppt |Week 8 progress update]]
 
*[[Media:Cyanobacteria_presentation_Week_9.ppt |Week 9 progress update]]
 
*[[Media:Cyanobacteria_presentation_Week_9.ppt |Week 9 progress update]]
*[[Media:Cyanobacteria_presentation_Week_9.ppt |Week 10 progress update, 50% complete]]
+
*[[Media:Cyanobacteria_presentation_Week_10.ppt |Week 10 progress update, 50% complete]]
 +
 
 
==Team Members==
 
==Team Members==
 
*[[User:Hetmann|Hetmann Hsieh]] ([[User_talk:Hetmann|talk]], [[Special:Contributions/Hetmann|edits]])
 
*[[User:Hetmann|Hetmann Hsieh]] ([[User_talk:Hetmann|talk]], [[Special:Contributions/Hetmann|edits]])

Revision as of 22:52, 20 August 2006

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Construct Planning

Constructs we plan to create.


Synthesis

Synthesis

Agenda

See image at right for our long-term project outline.

Long-term project outline

Click here for our current agenda

Kai Gene sizes

  • KaiA size: 855bp
    • 903bp with BioBrick ends
  • KaiB: 309bp
    • 357bp with BioBrick ends
  • KaiC: 1560bp

BioBricks Used

  • <bbpart>BBa_J04450</bbpart>
    • RFP device
    • Insert size: 1069bp
    • [pSB1A2]
      • High-copy, AmpR
      • Size: 2079bp
  • <bbpart>BBa_J04500</bbpart>
    • Lac promoter + RBS
    • Insert size: 220bp
    • [pSB1AK3]
      • High-copy, AmpR, KanR
      • Insert size: 3189bp
  • [pSB4A3]
    • Low-copy, AmpR
    • Insert size: 3339 bp
  • <bbpart>BBa_R0010</bbpart> + <bbpart>BBa_E0241</bbpart>
    • GFP device
    • Insert size: 995 bp

Presentations

Team Members

Recent Changes

19 April 2018

     15:50  Luckau Protocols:TAE‎ (diff | hist) . . (+47). . Tara K. Luckau (talk | contribs) (To make 500mL of 50x TAE)

     12:31 

XROMM‎‎ (8 changes | history) . . (+1,185). . [Aolsen‎ (8×)]

     

12:31

(cur | prev) . . (+57). . Aolsen (talk | contribs)

     

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(cur | prev) . . (0). . Aolsen (talk | contribs)

     

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(cur | prev) . . (0). . Aolsen (talk | contribs)

     

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(cur | prev) . . (+55). . Aolsen (talk | contribs)

     

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(cur | prev) . . (+273). . Aolsen (talk | contribs)

     

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(cur | prev) . . (+364). . Aolsen (talk | contribs)

     

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(cur | prev) . . (+108). . Aolsen (talk | contribs)

     

11:48

(cur | prev) . . (+328). . Aolsen (talk | contribs)

     11:40 

Silver: 2018 Meeting Schedule‎‎ (4 changes | history) . . (+6). . [PamSilver‎ (4×)]

     

11:40

(cur | prev) . . (+2). . PamSilver (talk | contribs) (June)

     

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(cur | prev) . . (0). . PamSilver (talk | contribs)

     

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(cur | prev) . . (+1). . PamSilver (talk | contribs) (May)

     

11:38

(cur | prev) . . (+3). . PamSilver (talk | contribs) (April)

     11:26 (Move log) . . Aolsen (talk | contribs) moved page Xromm to XROMM(Acronym looks better in caps)

N    10:58 

Xromm‎‎ (2 changes | history) . . (+708). . [Aolsen‎ (2×)]

     

10:58

(cur | prev) . . (-767). . Aolsen (talk | contribs)

N    

10:57

(cur | prev) . . (+1,475). . Aolsen (talk | contribs) (Created page with "{{back to protocols}} X-ray Reconstruction of Moving Morphology (XROMM) is a 3D imaging technology, developed at Brown University, for visualizing rapid skeletal movement in v...")

     08:40  Taylor:Publications‎ (diff | hist) . . (+18). . Lynne S. Taylor (talk | contribs) (PUBLICATIONS)
     07:22  Lee:JC‎ (diff | hist) . . (+166). . Wooin Lee (talk | contribs) (Lab meeting schedule)

18 April 2018

     20:43 

X-Lab‎‎ (4 changes | history) . . (0). . [ChanEngr‎ (4×)]

     

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(cur | prev) . . (0). . ChanEngr (talk | contribs)

     

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(cur | prev) . . (0). . ChanEngr (talk | contribs)

     

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(cur | prev) . . (0). . ChanEngr (talk | contribs)

 m   16:47 

User:Korey Griffin‎‎ (2 changes | history) . . (+32). . [Korey Griffin‎ (2×)]

 m   

16:47

(cur | prev) . . (-47). . Korey Griffin (talk | contribs) (Contact)

 m   

11:03

(cur | prev) . . (+79). . Korey Griffin (talk | contribs) (Empirical Resources)

     15:21 

User:Johannes Stuttmann‎‎ (3 changes | history) . . (+4,407). . [Johannes Stuttmann‎ (3×)]

     

15:21

(cur | prev) . . (+1,692). . Johannes Stuttmann (talk | contribs)

     

14:42

(cur | prev) . . (+2,470). . Johannes Stuttmann (talk | contribs)

     

13:09

(cur | prev) . . (+245). . Johannes Stuttmann (talk | contribs)

     13:02  Hydrochloric acid‎ (diff | hist) . . (+1). . Yar (talk | contribs) (typing error from user email)

     12:52 

(User creation log). .

[Yar‎ (2×)]

     

12:52

. . User account Ally B (talk | contribs) was created by Yar (talk | contribs) and password was sent by email ‎

     

12:52

. . User account Jakob (talk | contribs) was created by Yar (talk | contribs) and password was sent by email ‎

     12:46 

BIOL478/S18:Microarray Data Analysis‎‎ (20 changes | history) . . (+7,439). . [Kam D. Dahlquist‎ (20×)]

     

12:46

(cur | prev) . . (-70). . Kam D. Dahlquist (talk | contribs) (Gridding and Generating Intensity Data: remove old Brightspace link)

     

12:45

(cur | prev) . . (+1). . Kam D. Dahlquist (talk | contribs) (GenePix Pro Protocol (Steps 1-3): fix Brightspace link to raw data)

     

12:16

(cur | prev) . . (+1,241). . Kam D. Dahlquist (talk | contribs) (Before We Begin: add section on lab notebook)

     

12:04

(cur | prev) . . (0). . Kam D. Dahlquist (talk | contribs) (Background: fix list formatting)

     

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(cur | prev) . . (+108). . Kam D. Dahlquist (talk | contribs) (Background: add GRNsight)

     

12:02

(cur | prev) . . (+3,845). . Kam D. Dahlquist (talk | contribs) (Identifying regulatory transcription factors responsible for observed changes in gene expression (step 10): paste in GRNsight instructions)

     

12:00

(cur | prev) . . (+3,072). . Kam D. Dahlquist (talk | contribs) (Identifying regulatory transcription factors responsible for observed changes in gene expression (step 10): paste in protocol from Biomath modeling, modify for HMO1)

     

11:56

(cur | prev) . . (+114). . Kam D. Dahlquist (talk | contribs) (Clustering and GO Term Enrichment with stem (steps 7-8): add section for step 10)

     

11:55

(cur | prev) . . (-751). . Kam D. Dahlquist (talk | contribs) (Clustering and GO Term Enrichment with stem (steps 7-8): delete comment, emphasize to unzip)

     

11:52

(cur | prev) . . (0). . Kam D. Dahlquist (talk | contribs) (Modified t test for each timepoint: fix formula)

     

11:29

(cur | prev) . . (+52). . Kam D. Dahlquist (talk | contribs) (Sanity Check: Number of genes significantly changed: add label to link)

     

11:29

(cur | prev) . . (+29). . Kam D. Dahlquist (talk | contribs) (Sanity Check: Number of genes significantly changed: add link to xkcd comic)

     

11:25

(cur | prev) . . (-360). . Kam D. Dahlquist (talk | contribs) (replace opi1 with hmo1)

     

11:24

(cur | prev) . . (+18). . Kam D. Dahlquist (talk | contribs) (replace wt with dHMO1)

     

11:22

(cur | prev) . . (+36). . Kam D. Dahlquist (talk | contribs) (Modified t test for each timepoint: replace wt with dHMO1)

     

11:20

(cur | prev) . . (-22). . Kam D. Dahlquist (talk | contribs) (Looking at the raw data: fix links to SGD)

     

11:18

(cur | prev) . . (+3). . Kam D. Dahlquist (talk | contribs) (Downloading the Data: change to hmo1)

     

11:07

(cur | prev) . . (+35). . Kam D. Dahlquist (talk | contribs) (Statistical Analysis (Step 6): change to delta-hmo1)

     

10:35

(cur | prev) . . (+61). . Kam D. Dahlquist (talk | contribs) (GenePix Pro Protocol (Steps 1-3): note that this will be demo'd, change opi1 to hmo1)

     

10:33

(cur | prev) . . (+27). . Kam D. Dahlquist (talk | contribs) (Background: steps 1-3 done for you)