IGEM:Groningen/Notebook/iGEM 2011/2011/06/28: Difference between revisions
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Joyce Mulder (talk | contribs) (Autocreate 2011/06/28 Entry for IGEM:Groningen/Notebook/iGEM_2011) |
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== | ==28-6-11== | ||
<br> | |||
<br> Colony PCR PhybB RBS GFP DT | |||
<br> Taq 10× buffer: 20μl | |||
<br> dNTPs 10mM: 4μl | |||
<br> MgCl2: 12μl | |||
<br> Forward Biobrick primer: 4μl | |||
<br> Reverse Biobrick primer: 4μl | |||
<br> Taq DNA polymerase: 1μl | |||
<br> MQ water: 155μl | |||
<br> | |||
<br> PCR conditions: | |||
<br> Preheated lid: 111°C | |||
<br> Denaturation: 94°C for 10 min. | |||
<br> Cycle 33×: | |||
<br> denaturation: 94°C for 30s. | |||
<br> annealing: 60°C for 30s. | |||
<br> Extension: 72°C for 2,5 min. | |||
<br> Final extension: 72°C for 10 min. | |||
<br> Store infinite at 4°C | |||
<br> | |||
<br> Analyse on a 1% TBE agarose gel. | |||
<br> | |||
<br> Making plan for parallel cloning work | |||
<br> | |||
<br> Helping with sponsoring and doing some Human practices work | |||
<br> | |||
<br> | |||
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Revision as of 03:49, 21 September 2011
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28-6-11
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