Difference between revisions of "IGEM:Groningen/Notebook/iGEM 2011/2011/06/09"

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(Autocreate 2011/06/09 Entry for IGEM:Groningen/Notebook/iGEM_2011)
 
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==Entry title==
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==09-06-11==
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<br>
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<br>Transformation
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<br>Add to 40μl competent cells 10μl ligation mixture
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<br>Incubate for 30 min on ice
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<br>Heat shock: 45s at 42 degrees
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<br>Incubate cells on ice for 2 min
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<br>Add 1ml LB medium+ 25mM glucose
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<br>Incubate cells for 1h/1.5h at 37 degrees
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<br>Spin the cells down, resuspend them in 100μl LB medium and plate 90μl and 10μl out on the plates
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<br>Put the plates in the stove at 37 degrees overnight
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<br>
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<br>Meanwhile, prepare the plates with ampicillin and kanamycin
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<br>Work on Human practices and report+ researchplan
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<br>Teammeeting at 5!
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<br>
 
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09-06-11



Transformation
Add to 40μl competent cells 10μl ligation mixture
Incubate for 30 min on ice
Heat shock: 45s at 42 degrees
Incubate cells on ice for 2 min
Add 1ml LB medium+ 25mM glucose
Incubate cells for 1h/1.5h at 37 degrees
Spin the cells down, resuspend them in 100μl LB medium and plate 90μl and 10μl out on the plates
Put the plates in the stove at 37 degrees overnight

Meanwhile, prepare the plates with ampicillin and kanamycin
Work on Human practices and report+ researchplan
Teammeeting at 5!