IGEM:Groningen/Notebook/iGEM 2011/2011/06/01

From OpenWetWare
Revision as of 01:29, 21 September 2011 by Joyce Mulder (talk | contribs) (Entry title)
Jump to: navigation, search
Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


Do a PCR for PhybB on E.coli DH5alpha suspension:
Taq 10× buffer: 5μl
dNTPs: 1μl
MgCl2: 3μl
taq 5u/μl: 0.25μl
Forward primer 10mM: 1μl
Reverseprimer 10mM: 1μl
Template: 1μl
MQ: 38.75μl

Analyse them on a 1% agarosegel
PCR conditions:
denaturation: 10min
Cycle 25×
denaturtaion: 1min
annealing: 1min (gradient for 60, 65 and 70 degrees)
extension: 1min
Final extension: 10min
Store infinite

Making cloning strategy with biobrick parts