Difference between revisions of "IGEM:Cambridge/2008/Notebook/Voltage/2008/09/16"

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(Autocreate 2008/09/16 Entry for IGEM:Cambridge/2008/Notebook/Voltage)
 
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==Entry title==
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==Ligations==
* Insert your content here.
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* GluR0 + pSB4T5 or pSB3T5
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* B1002 + B1006
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==Gel==
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* B1002 + B1006 ligation product run with GluR0 unsupercoiled
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** GluR0 was lightly irradiated to induce sing strand breaks to allow it to de-supercoil and act as a 4kb template.
  
 
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Revision as of 08:25, 3 October 2008



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Ligations

  • GluR0 + pSB4T5 or pSB3T5
  • B1002 + B1006

Gel

  • B1002 + B1006 ligation product run with GluR0 unsupercoiled
    • GluR0 was lightly irradiated to induce sing strand breaks to allow it to de-supercoil and act as a 4kb template.