Difference between revisions of "IGEM:Cambridge/2008/Notebook/Voltage/2008/08/26"

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==Kdp screening==
 
==Kdp screening==
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* Single colony PCR was perfomed over a selection of 44 colonies using forward and reverse biobrick primers in order to gauge the size of the vector insert by gel visualisation.
 
* Single colony PCR was perfomed over a selection of 44 colonies using forward and reverse biobrick primers in order to gauge the size of the vector insert by gel visualisation.
 
*All screened colonies were negative for Kdp - concluded most likely to be blunt ended self ligation of pSB4C5 vector (without death gene).  
 
*All screened colonies were negative for Kdp - concluded most likely to be blunt ended self ligation of pSB4C5 vector (without death gene).  
 
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Revision as of 07:20, 3 September 2008



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Kdp screening

  • Many transformant colonies were found on chloramphenicol plates.
  • Single colony PCR was perfomed over a selection of 44 colonies using forward and reverse biobrick primers in order to gauge the size of the vector insert by gel visualisation.
  • All screened colonies were negative for Kdp - concluded most likely to be blunt ended self ligation of pSB4C5 vector (without death gene).