IGEM:Cambridge/2008/Notebook/Voltage

From OpenWetWare
Jump to: navigation, search

<!-- sibboleth --><div id="lncal1" style="border:0px;"><div style="display:none;" id="id">lncal1</div><div style="display:none;" id="dtext">07/15/2008,07/16/2008,07/17/2008,07/18/2008,07/21/2008,07/22/2008,07/23/2008,07/24/2008,07/25/2008,07/28/2008,07/29/2008,07/30/2008,07/31/2008,08/01/2008,08/04/2008,08/05/2008,08/06/2008,08/07/2008,08/08/2008,08/11/2008,08/12/2008,08/13/2008,08/14/2008,08/15/2008,08/18/2008,08/19/2008,08/20/2008,08/21/2008,08/22/2008,08/25/2008,08/26/2008,08/27/2008,08/28/2008,08/29/2008,09/02/2008,09/03/2008,09/04/2008,09/05/2008,09/08/2008,09/09/2008,09/10/2008,09/11/2008,09/12/2008,09/15/2008,09/16/2008,09/17/2008,09/18/2008,09/19/2008,09/22/2008,09/23/2008,09/24/2008,09/25/2008,09/26/2008,09/29/2008,09/30/2008</div><div style="display:none;" id="page">IGEM:Cambridge/2008/Notebook/Voltage</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month"></div><div style="display:none;" id="year"></div><div style="display:none;" id="readonly">Y</div></div>

Untitled-2.jpg <sitesearch>title=Search this project</sitesearch>

Customize your entry pages <html><img src="/images/a/aa/Help.png" border="0" /></html>

Aim

To create a system which responds to ligand binding with a detectable voltage caused by a K+ flux.

Roadmap

Background

Presentation

Experiments

Mutant Strains

Flame Photometer Calibration

[[K+ Concentrations]]

BioBrick Manipulation

OD600 Calibration

Next Steps

Characterise promoters

1. Simulate and design ‘reporter plasmids’ with correct biobricks restriction enzyme sites

2. For each strain: 2 plasmid backbones

  • PSC101 (A) with OsmY (promoter) + RFP + Stop
  • Another (B), not yet defined, with Bba_J23100 + YFP + Stop
Tests for each strain : plasmid A, plasmid B, plasmid A + plasmid B (so 15 tests)

3. Quantify transformation efficiency (colony counter)

4. Quantify promoter strength (light intensity, expression levels)

Useful Links

Protein prediction tools

Uniprot database

Literature

Kdp operon diagram

Kdp plasmid

The Kdp-ATPase system and its regulation

Potential Chassis: Strain JW1242-1 Strain JW0710-1

Kdp mutant - paper from 1971

Worldwide E.coli Databases

Characterisation of kdpD - 2005

Investigations on Kdp Operon exp. & flux

Very interesting 2001 paper concerning Glutamate Channels

1999 paper on functional characterization of prokaryote Glu Channels

Sequenced Synechocystis PCC 6803 genome

Glutamate-gated K+ channel GluR0

Link to E.coli statistics page (CCDB Database)

Recent changes

18 October 2017

     11:37 

BME100 f2017:Group5 W1030 L4‎‎ (3 changes | history) . . (+42). . [Sarah Nystrom‎; Thomas J. On‎ (2×)]

     

11:37

(cur | prev) . . (+39). . Thomas J. On (talk | contribs) (Research and Development)

     

11:32

(cur | prev) . . (-2). . Thomas J. On (talk | contribs) (Research and Development)

     

11:28

(cur | prev) . . (+5). . Sarah Nystrom (talk | contribs) (Protocol)

     11:37 

BME100 f2017:Group12 W1030 L4‎‎ (3 changes | history) . . (+749). . [Alec McCall‎; Jasmine C. Barboa‎ (2×)]

     

11:37

(cur | prev) . . (+2). . Jasmine C. Barboa (talk | contribs) (OUR TEAM)

     

11:36

(cur | prev) . . (+728). . Alec McCall (talk | contribs) (Research and Development)

     

11:32

(cur | prev) . . (+19). . Jasmine C. Barboa (talk | contribs) (OUR TEAM)

     11:36  BME100 f2017:Group9 W1030 L4‎ (diff | hist) . . (+10). . Carlos I. Ruiz Jr. (talk | contribs) (OUR TEAM)

     11:35 

(Upload log). .

[Sarah Desmond‎ (2×); Nathan O. Moorman‎ (2×); Jacob Tomas Harrison Haye‎ (2×); Carlos I. Ruiz Jr.‎ (2×); Jasmine C. Barboa‎ (3×); Alexia Kamau‎ (3×)]

     

11:35

. . Jasmine C. Barboa (talk | contribs) uploaded File:BpVOIqaIQAANTZp.jpg

     

11:35

. . Jacob Tomas Harrison Haye (talk | contribs) uploaded a new version of File:Tom-holland-spider-man.jpg

     

11:33

. . Alexia Kamau (talk | contribs) uploaded a new version of File:Dna Strand Group16.jpeg

     

11:33

. . Carlos I. Ruiz Jr. (talk | contribs) uploaded a new version of File:Charlie Brown x MF DOOM.jpg

     

11:33

. . Carlos I. Ruiz Jr. (talk | contribs) uploaded File:Charlie Brown x MF DOOM.jpg

     

11:33

. . Alexia Kamau (talk | contribs) uploaded a new version of File:Dna Strand Group16.jpeg

     

11:33

. . Jasmine C. Barboa (talk | contribs) uploaded File:635802796596030866-909315103 JIMMY.png

     

11:31

. . Jacob Tomas Harrison Haye (talk | contribs) uploaded File:Tom-holland-spider-man.jpg

     

11:31

. . Alexia Kamau (talk | contribs) uploaded a new version of File:Dna Strand Group16.jpeg

     

11:30

. . Nathan O. Moorman (talk | contribs) uploaded a new version of File:Pickle.jpg

     

11:29

. . Sarah Desmond (talk | contribs) uploaded File:Lesliedrinkingcoffee.jpeg

     

11:29

. . Jasmine C. Barboa (talk | contribs) uploaded File:636221410316183057-A01-v2-REAR17.jpg

     

11:28

. . Sarah Desmond (talk | contribs) uploaded File:Patrickbitmoki---.jpeg

     

11:27

. . Nathan O. Moorman (talk | contribs) uploaded File:Pickle.jpg

     11:35 

BME100 f2017:WikiHelp‎‎ (3 changes | history) . . (+336). . [Karmella Haynes‎ (3×)]

     

11:35

(cur | prev) . . (+126). . Karmella Haynes (talk | contribs) (Text Editing)

     

11:30

(cur | prev) . . (+168). . Karmella Haynes (talk | contribs) (Text Editing)

     

11:29

(cur | prev) . . (+42). . Karmella Haynes (talk | contribs) (Text Editing)

     11:35 

BME100 f2017:Group4 W1030 L4‎‎ (3 changes | history) . . (+1). . [Alexis A. Chavez‎ (3×)]

     

11:35

(cur | prev) . . (+1). . Alexis A. Chavez (talk | contribs) (OUR TEAM)

     

11:35

(cur | prev) . . (-2). . Alexis A. Chavez (talk | contribs) (OUR TEAM)

     

11:32

(cur | prev) . . (+2). . Alexis A. Chavez (talk | contribs) (OUR TEAM)

     11:33 

BME100 f2017:Group10 W1030 L4‎‎ (3 changes | history) . . (+444). . [Adrienne L. Parkinson‎; Sarah Desmond‎ (2×)]

     

11:33

(cur | prev) . . (-35). . Sarah Desmond (talk | contribs) (OUR TEAM)

     

11:30

(cur | prev) . . (+33). . Sarah Desmond (talk | contribs) (OUR TEAM)

     

11:27

(cur | prev) . . (+446). . Adrienne L. Parkinson (talk | contribs) (Protocol)

     11:33 

BME100 f2017:Group2 W1030 L4‎‎ (2 changes | history) . . (-10). . [Kmwalke8‎ (2×)]

     

11:33

(cur | prev) . . (-7). . Kmwalke8 (talk | contribs) (Protocol)

     

11:30

(cur | prev) . . (-3). . Kmwalke8 (talk | contribs) (Protocol)

     11:32 

BME100 f2017:Group1 W1030 L4‎‎ (3 changes | history) . . (+265). . [Abby E. Krell‎ (3×)]

     

11:32

(cur | prev) . . (+6). . Abby E. Krell (talk | contribs) (Protocol)

     

11:31

(cur | prev) . . (+1). . Abby E. Krell (talk | contribs) (Protocol)

     

11:28

(cur | prev) . . (+258). . Abby E. Krell (talk | contribs) (Protocol)

     11:31  BME100 f2017:Group16 W1030 L4‎ (diff | hist) . . (+194). . Michelle M Mungaray (talk | contribs)

N    11:31 

BME100 f2017:Group13 W1030 L4‎‎ (3 changes | history) . . (+5,015). . [Brenna S. Toshner‎; Matthew K Castile‎ (2×)]

     

11:31

(cur | prev) . . (+30). . Matthew K Castile (talk | contribs) (Protocol)

     

11:30

(cur | prev) . . (+333). . Brenna S. Toshner (talk | contribs) (Protocol)

N    

11:28

(cur | prev) . . (+4,652). . Matthew K Castile (talk | contribs) (Created page with "{|{{table}} width="800" |- |style="background-color: #EEE"|128px<span style="font-size:22px;"> BME 100 Fall 2017</span> |style="background-color...")

     11:31 

Yeo lab:Contact‎‎ (3 changes | history) . . (+34). . [Yoonyeo‎ (3×)]

     

11:31

(cur | prev) . . (+16). . Yoonyeo (talk | contribs) (Direction to RHPH (Weekdays))

     

11:29

(cur | prev) . . (+9). . Yoonyeo (talk | contribs) (Lab office (RHPH G22))

     

11:29

(cur | prev) . . (+9). . Yoonyeo (talk | contribs) (Lab (RHPH G12))

     11:30  BME100 f2017:Group15 W1030 L4‎ (diff | hist) . . (+153). . Lauren N. Seliger (talk | contribs) (Lab B: Disease SNP - Specific Primer Design)
     11:30  BME100 f2017:Group14 W1030 L4‎ (diff | hist) . . (+34). . Lydia J Gabric (talk | contribs) (Protocol)
     11:30  BME100 f2017:Group8 W1030 L4‎ (diff | hist) . . (+9). . Jacob Tomas Harrison Haye (talk | contribs) (OUR TEAM)

     11:29 

BME100 f2017:Group11 W1030 L4‎‎ (2 changes | history) . . (+479). . [Zoe Marmitt‎ (2×)]

     

11:29

(cur | prev) . . (+3). . Zoe Marmitt (talk | contribs) (Research and Development)

     

11:28

(cur | prev) . . (+476). . Zoe Marmitt (talk | contribs) (Research and Development)

     11:28  BME100 f2017:Group13 W1030 L5‎ (diff | hist) . . (-432). . Brenna S. Toshner (talk | contribs) (Materials)

     11:28 

BME100 f2017:Group3 W1030 L4‎‎ (2 changes | history) . . (-8). . [Michael A. Finocchiaro‎ (2×)]

     

11:28

(cur | prev) . . (+1). . Michael A. Finocchiaro (talk | contribs) (OUR TEAM)

     

11:28

(cur | prev) . . (-9). . Michael A. Finocchiaro (talk | contribs) (OUR TEAM)