Difference between revisions of "IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/09/15"

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(Entry title)
(Check big stock of RBS S and W)
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- Nanodrop
 
- Nanodrop
 +
 +
{|class="wikitable" style="text-align:center" border="1"
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|-
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! product !! concentration (ng/μL) !! 260/280
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|-
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| RBS W2 || 90.3 || 1.70
 +
|-
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| RBS W4 || 124.0 || 1.80
 +
|}
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 +
- Single digest with EcoRI, XbaI, SpeI and PstI
 +
 +
- Gel
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*Lane1 : HyperladderI
 +
*Lane2 : RBS S6 cut with EcoRI
 +
*Lane3 : RBS S6 cut with SpeI
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*Lane4 : RBS S6 cut with PstI
 +
*Lane5 : RBS S6 cut with XbaI
 +
*Lane6 : RBS S6 uncut
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*Lane7 : RBS W6 cut with EcoRI
 +
*Lane8 : RBS W6 cut with SpeI
 +
*Lane9 : RBS W6 cut with PstI
 +
*Lane10 : RBS W6 cut with XbaI
 +
*Lane11 : RBS W6 uncut
 +
*Lane12 : supercoiled ladder
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 +
*Result
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 +
  
  

Revision as of 03:20, 16 September 2008



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Check big stock of RBS S and W

  • We want to check our big stock of RBS S and W, with all digest enzymes

- Plasmid miniprep RBS S6 and RBS W6 (from 200mL flask)

- Nanodrop

product concentration (ng/μL) 260/280
RBS W2 90.3 1.70
RBS W4 124.0 1.80

- Single digest with EcoRI, XbaI, SpeI and PstI

- Gel

  • Lane1 : HyperladderI
  • Lane2 : RBS S6 cut with EcoRI
  • Lane3 : RBS S6 cut with SpeI
  • Lane4 : RBS S6 cut with PstI
  • Lane5 : RBS S6 cut with XbaI
  • Lane6 : RBS S6 uncut
  • Lane7 : RBS W6 cut with EcoRI
  • Lane8 : RBS W6 cut with SpeI
  • Lane9 : RBS W6 cut with PstI
  • Lane10 : RBS W6 cut with XbaI
  • Lane11 : RBS W6 uncut
  • Lane12 : supercoiled ladder
  • Result