Difference between revisions of "IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/09/15"
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(→Check big stock of RBS S and W) |
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- Nanodrop | - Nanodrop | ||
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| + | {|class="wikitable" style="text-align:center" border="1" | ||
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| + | ! product !! concentration (ng/μL) !! 260/280 | ||
| + | |- | ||
| + | | RBS W2 || 90.3 || 1.70 | ||
| + | |- | ||
| + | | RBS W4 || 124.0 || 1.80 | ||
| + | |} | ||
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| + | - Single digest with EcoRI, XbaI, SpeI and PstI | ||
| + | |||
| + | - Gel | ||
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| + | *Lane1 : HyperladderI | ||
| + | *Lane2 : RBS S6 cut with EcoRI | ||
| + | *Lane3 : RBS S6 cut with SpeI | ||
| + | *Lane4 : RBS S6 cut with PstI | ||
| + | *Lane5 : RBS S6 cut with XbaI | ||
| + | *Lane6 : RBS S6 uncut | ||
| + | *Lane7 : RBS W6 cut with EcoRI | ||
| + | *Lane8 : RBS W6 cut with SpeI | ||
| + | *Lane9 : RBS W6 cut with PstI | ||
| + | *Lane10 : RBS W6 cut with XbaI | ||
| + | *Lane11 : RBS W6 uncut | ||
| + | *Lane12 : supercoiled ladder | ||
| + | |||
| + | *Result | ||
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| + | |||
Revision as of 03:20, 16 September 2008
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Check big stock of RBS S and W
- Plasmid miniprep RBS S6 and RBS W6 (from 200mL flask) - Nanodrop
- Single digest with EcoRI, XbaI, SpeI and PstI - Gel
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