IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/09/13: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| Line 22: | Line 22: | ||
- Nanodrop | - Nanodrop | ||
{|class="wikitable" style="text-align:center" border="1" | |||
|- | |||
! product !! concentration (ng/μL) !! 260/280 !! quantity of DNA to add to have about 300ng (μL) !! added SDW for single digest (μL) | |||
|- | |||
| RBS W2 || 42.4 || 1.62 || 8 || 9 | |||
|- | |||
| RBS W4 || 35.1 || 1.61 || 10 || 7 | |||
|- | |||
| RBS W6 || 41.0 || 1.67 || 8 || 9 | |||
|- | |||
| RBS W8 || 72.8 || 1.61 || 5 || 12 | |||
|- | |||
| RBS W10 || 51.0 || 1.60 || 6 || 11 | |||
|} | |||
Revision as of 02:51, 16 September 2008
 |
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||
Grow RBS S6- Put the 10mL of LB with RBS S6 from yesterday into 200mL of LB with antibiotic - Incubate at 37°C with shaking RBS W screening- Plasmid miniprep of RBS W2, 4, 6, 8, 10 9witouh endo wash buffer) - Nanodrop
| |||||||||||||||||||||||||||||||
