Difference between revisions of "IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/09/13"

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(Entry title)
(RBS W screening)
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- Nanodrop
 
- Nanodrop
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{|class="wikitable" style="text-align:center" border="1"
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|-
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! product !! concentration (ng/μL) !! 260/280 !! quantity of DNA to add to have about 300ng (μL) !! added SDW for single digest (μL)
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|-
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| RBS W2 || 42.4 || 1.62 || 8 || 9
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|-
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| RBS W4 || 35.1 || 1.61 || 10 || 7
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|-
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| RBS W6 || 41.0 || 1.67 || 8 || 9
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|-
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| RBS W8 || 72.8 || 1.61 || 5 || 12
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|-
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| RBS W10 || 51.0 || 1.60 || 6 || 11
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|}
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Revision as of 01:51, 16 September 2008



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Grow RBS S6

- Put the 10mL of LB with RBS S6 from yesterday into 200mL of LB with antibiotic

- Incubate at 37°C with shaking

RBS W screening

- Plasmid miniprep of RBS W2, 4, 6, 8, 10 9witouh endo wash buffer)

- Nanodrop

product concentration (ng/μL) 260/280 quantity of DNA to add to have about 300ng (μL) added SDW for single digest (μL)
RBS W2 42.4 1.62 8 9
RBS W4 35.1 1.61 10 7
RBS W6 41.0 1.67 8 9
RBS W8 72.8 1.61 5 12
RBS W10 51.0 1.60 6 11