IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/09/12: Difference between revisions
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*Lane11 : RBS W | *Lane11 : RBS W | ||
*Lane12 : HyperladderI | *Lane12 : HyperladderI | ||
*Results | |||
[[Image:gel21091pcr.gif|200px|center]] | [[Image:gel21091pcr.gif|200px|center]] | ||
- agr B 2 bands? | |||
- agrC ok | |||
- rep small band but good size | |||
- backbones : ok | |||
- promoters : ok | |||
- RBS : nothing | |||
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Revision as of 02:33, 16 September 2008
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RBS screening (single digest)- Plasmid miniprep 6 colonies of RBS S and 6 colonies of RBS W (without endo wash buffer) - Nanodrop
- Single digest : with EcoRI and XbaI, add SDW and DNA (according to the previous table, 300mg of DNA), 2μL of fast digest buffer, and 1μL of enzyme - Gel Check PCR products- Gel (2μL of each product)
- agr B 2 bands? - agrC ok - rep small band but good size - backbones : ok - promoters : ok - RBS : nothing |