Difference between revisions of "IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/09/12"

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(Check PCR products)
(RBS screening (single digest))
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==RBS screening (single digest)==
 
==RBS screening (single digest)==
  
-
+
- Plasmid miniprep 6 colonies of RBS S and 6 colonies of RBS W
 +
 
 +
- Nanodrop
  
 
==Check PCR products==
 
==Check PCR products==

Revision as of 01:16, 16 September 2008



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RBS screening (single digest)

- Plasmid miniprep 6 colonies of RBS S and 6 colonies of RBS W

- Nanodrop

Check PCR products

- Gel (2μL of each product)

  • Lane1 : λ ladder
  • Lane2 : agrA
  • Lane3 : agrB
  • Lane4 : agrC
  • Lane5 : rep
  • Lane6 : pSB4C5 1
  • Lane7 : pSB4C5 2
  • Lane8 : Pxyl
  • Lane9 : Ppac
  • Lane10 : RBS S
  • Lane11 : RBS W
  • Lane12 : HyperladderI
Gel21091pcr.gif