Difference between revisions of "IGEM:Cambridge/2008/Notebook/Magnetic Bacteria/2008/08/06"

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! [Fe]/μM !! 0 !! 4 !! 8 !! 12 !! 16 !! 20
 
! [Fe]/μM !! 0 !! 4 !! 8 !! 12 !! 16 !! 20

Revision as of 04:30, 7 August 2008



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Getting Promoter Plasmid and Iron Assay with Fur- Mutants

Iron Assay with Fur- Mutants

Protocol for Varying Iron Concentration in Medium
[Fe]/μM 0 4 8 12 16 20
Vol. of Fe stock/ml 0 0.01 0.02 0.03 0.04 0.05
Vol. of SDW/ml 0.50 0.49 0.48 0.47 0.46 0.45
Vol. of fur- cells in M9/ml 9.5 9.5 9.5 9.5 9.5 9.5