IGEM:Cambridge/2008/Notebook/Bacillus/2008/09/09: Difference between revisions
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*Result | *Result | ||
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- No insert in IA751 transformed with ECE153 and ECE112 (same size than control!) and however the amylase test is positive for this colony.... | - No insert in IA751 transformed with ECE153 and ECE112 (same size than control!) and however the amylase test is positive for this colony.... |
Revision as of 03:43, 16 September 2008
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Chromosomal DNA extraction- ZR soil microbe DNA kit for
- Nanodrop
- Gel
- Only something for IA751, and ECE153 (pure plasmid)??? - New PCR with 4μL of chromosomal DNA and new PCR program - New gel
- No insert in IA751 transformed with ECE153 and ECE112 (same size than control!) and however the amylase test is positive for this colony.... PCR AmyE again- 24μL of SDW, 10μL of MM, 2μL of each primers, 2μL of ECE112
- Very good result for amyE front, nothing for AmyE back |