Difference between revisions of "IGEM:Cambridge/2008/Notebook/Bacillus"

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=Testing ''B.subtilis'' promoters and RBSs=
 
=Testing ''B.subtilis'' promoters and RBSs=
 
We are seeking to test the expression strength and response to inducers in several promoters in ''B.subtilis'', in combination with different Bacillus-specific ribosome binding sites.  
 
We are seeking to test the expression strength and response to inducers in several promoters in ''B.subtilis'', in combination with different Bacillus-specific ribosome binding sites.  
 +
 +
=B. subtilis Promoter Testing with Beta-galactosidase Assay=
 +
 +
We are aiming to characterize four different Bacillus subtilis promoters using the beta-galactosidase assay. (Protocol for beta-galactosidase assay as described [http://openwetware.org/wiki/Beta-Galactosidase_Assay_(A_better_Miller) here]
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 +
B. subtilis Promoters to be Tested:
 +
* Pupp
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* Ppac
 +
* Pspac
 +
* Pxyl
 +
 +
  
 
=Links=
 
=Links=

Revision as of 08:20, 3 September 2008



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Testing B.subtilis promoters and RBSs

We are seeking to test the expression strength and response to inducers in several promoters in B.subtilis, in combination with different Bacillus-specific ribosome binding sites.

B. subtilis Promoter Testing with Beta-galactosidase Assay

We are aiming to characterize four different Bacillus subtilis promoters using the beta-galactosidase assay. (Protocol for beta-galactosidase assay as described here

B. subtilis Promoters to be Tested:

  • Pupp
  • Ppac
  • Pspac
  • Pxyl


Links

B. Subtilis Transformation Protcol - Electroporation

Bongers et al. … of a Subtilin-Regulated Expression System in Bacillus subtilis: Strict Control of Gene Expression …. Applied and Environmental Microbiology (2005)

  • SURE: handy expression system for B. subtilis
  • What about feedback/regulation effects of subtilin on the cell

Expression and characterization of aiiA gene from Bacillus subtilis BS-1.

  • (Some strains of) Bacillus subtilis produces a gene called aiiA that degrades AHL molecules from gram-negative bacteria
  • this is biobricked

Links to Cambridge 2007 Wiki