Difference between revisions of "IGEM:Caltech/2008/Project/Lactose intolerance"

From OpenWetWare
Jump to: navigation, search
 
Line 15: Line 15:
 
The general idea is to overexpress LacY (Lactose transporter) with LacZ (Beta-Galactosidase). When lactose is present, the cell will uptake lactose and will flip on the Lac promoter repressed by lacI when enough lactose is present. We then express lysozyme and holin which will lyse the cell releasing beta-gal cleaving lactose into glucose and galactose. Glucose and galactose will then be taken up by the host in the colon.<br>
 
The general idea is to overexpress LacY (Lactose transporter) with LacZ (Beta-Galactosidase). When lactose is present, the cell will uptake lactose and will flip on the Lac promoter repressed by lacI when enough lactose is present. We then express lysozyme and holin which will lyse the cell releasing beta-gal cleaving lactose into glucose and galactose. Glucose and galactose will then be taken up by the host in the colon.<br>
 
==[[/Lactose Outline|Lactose Outline]]==
 
==[[/Lactose Outline|Lactose Outline]]==
 +
 +
 +
 +
 +
 +
 +
|} </div>

Latest revision as of 14:52, 15 August 2008


Caltech phage.jpg

iGEM 2008

Home        People        Project        Protocols        Notes        Ideas        Changes       


Lactose Summary

We want to make two plasmids:
Plasmid #1 - A constitutive promoter with LacZ and LacY.
Plasmid #2 - A lactose inducible promoter with lysozyme and holin.
The general idea is to overexpress LacY (Lactose transporter) with LacZ (Beta-Galactosidase). When lactose is present, the cell will uptake lactose and will flip on the Lac promoter repressed by lacI when enough lactose is present. We then express lysozyme and holin which will lyse the cell releasing beta-gal cleaving lactose into glucose and galactose. Glucose and galactose will then be taken up by the host in the colon.

Lactose Outline