Difference between revisions of "IGEM:Caltech/2007"

About $\lambda$
Enterobacteriophage $\lambda$ is a temperate virus that infects Escherichia Coli cells. Once inside the cell, $\lambda$ chooses between two pathways. It can enter the lytic pathway, in which it uses host cell machinery to manufacture copies of itself and eventually releases them by lysing the cell. It can also enter the lysogenic pathway, in which it inserts its genome into that of the host where it is replicated along with the host genome. We want to manipulate $\lambda$'s decision in response to molecular signals inside the host cell, so that it only lyses a specific subpopulation of cells. If successful, this will give us more insight on how life cycle decisions are made in the $\lambda$ bacteriophage.
There are three control points in the $\lambda$ genome we want to regulate: cro, N, and Q. Using three control points gives us more control over $\lambda$'s life cycle, as well as providing us with redundancy in case one of the regulatory systems don't work.