IGEM:Brown/2008/Notebook/Team Resistance/2008/06/11: Difference between revisions
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==Team Resistance== | ==Team Resistance== | ||
* Our team is working to create a biosensor that detects the presence of a toxin via the change in resistance of the bacterial solution. | * Our team is working to create a biosensor that detects the presence of a toxin via the change in resistance of the bacterial solution. | ||
Testing apparatus with NaCl at different concentrations | |||
• 3 plates with 0.05M NaCl (0.02922g in 10mL) | |||
• 3 plates with 0.005M NaCl (0.02922g in 100mL) | |||
• 3 plates with 0.001M NaCl (0.01461g in 250mL) | |||
dilute to each concentration then put 10 mL of each solution in respective plates | |||
Control: distilled water | |||
Results: | |||
Control: 4.108Mohm, 2.517Mohm, 2.934Mohm | |||
0.001M NaCl: 393.7 kohm, 265.5kohm, 579.6kohm | |||
0.005M NaCl: 264.9kohm, 266kohm, 326.1kohm | |||
0.05M NaCl: 233.5kohm, 46.69kohm, 97.1kohm | |||
control 0.001M NaCl 0.005M NaCl 0.05M NaCl | |||
3.186MΩ 0.4129MΩ 0.2857MΩ 0.1258MΩ | |||
0.001M NaCl: 2.7731MΩ change | |||
0.005M NaCl: 2.9003MΩ change | |||
0.05M NaCl: 3.0602MΩ change | |||
• Our wires were not long enough to touch the solution in the plates—next time don’t tape wires in place until right before experiment begins | |||
• WE NEED A COMPUTER BASED VOLTMETER | |||
Questions for Dan Ludwig (Meeting Friday 3:00pm) | |||
Should touching the plastic significantly change the resistance? | |||
What if distance changes slightly? | |||
All considerations of our apparatus | |||
Any suggestions for a real-time, computer based voltmeter? | |||
Tomorrow: get plates from Harvard lab! | |||
Create glycerol stocks of the plasmids | |||
Revision as of 08:24, 16 June 2008
 iGEM Project name 1
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Team Resistance
Testing apparatus with NaCl at different concentrations • 3 plates with 0.05M NaCl (0.02922g in 10mL) • 3 plates with 0.005M NaCl (0.02922g in 100mL) • 3 plates with 0.001M NaCl (0.01461g in 250mL) dilute to each concentration then put 10 mL of each solution in respective plates Control: distilled water Results: Control: 4.108Mohm, 2.517Mohm, 2.934Mohm 0.001M NaCl: 393.7 kohm, 265.5kohm, 579.6kohm 0.005M NaCl: 264.9kohm, 266kohm, 326.1kohm 0.05M NaCl: 233.5kohm, 46.69kohm, 97.1kohm control 0.001M NaCl 0.005M NaCl 0.05M NaCl 3.186MΩ 0.4129MΩ 0.2857MΩ 0.1258MΩ 0.001M NaCl: 2.7731MΩ change 0.005M NaCl: 2.9003MΩ change 0.05M NaCl: 3.0602MΩ change • Our wires were not long enough to touch the solution in the plates—next time don’t tape wires in place until right before experiment begins • WE NEED A COMPUTER BASED VOLTMETER
Should touching the plastic significantly change the resistance? What if distance changes slightly? All considerations of our apparatus Any suggestions for a real-time, computer based voltmeter? Tomorrow: get plates from Harvard lab! Create glycerol stocks of the plasmids
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