Difference between revisions of "IGEM:Brown/2008/Notebook/Team Resistance/2008/06/08"

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(Considerations for gene cassette)
(Considerations for gene cassette)
Line 10: Line 10:
 
==Considerations for gene cassette==
 
==Considerations for gene cassette==
 
Harvard’s paper
 
Harvard’s paper
 +
 
• Used luria lb
 
• Used luria lb
  
Line 25: Line 26:
  
 
Materials for lab:
 
Materials for lab:
 +
 
More 1000ml wheaton bottles to be able to store lb
 
More 1000ml wheaton bottles to be able to store lb
 
(possibly borrow)
 
(possibly borrow)
  
 
Requested meeting with professor palmore
 
Requested meeting with professor palmore
 +
 
Questions
 
Questions
 +
 
• What kind of things affect resistance (in terms of our apparatus)
 
• What kind of things affect resistance (in terms of our apparatus)
  
  
-wires slipped through cardboard
+
wires slipped through cardboard
 +
 
 
• distance between each wire in plates 0.25 in. and 1.73 in. into plates
 
• distance between each wire in plates 0.25 in. and 1.73 in. into plates
  

Revision as of 08:21, 16 June 2008

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Team Resistance

  • Our team is working to create a biosensor that detects the presence of a toxin via the change in resistance of the bacterial solution.

Considerations for gene cassette

Harvard’s paper

• Used luria lb

• Grew cells up to mid-logarithmic stage then added the arabinose

• Saw decrease in cell density after just 1 hour, but no change in the control

• Used plasmid pvj4

• E.coli strains: DH5alpha Beta2155

• Can we check tightness of promoters by using gfp?

Materials for lab:

More 1000ml wheaton bottles to be able to store lb (possibly borrow)

Requested meeting with professor palmore

Questions

• What kind of things affect resistance (in terms of our apparatus)


wires slipped through cardboard

• distance between each wire in plates 0.25 in. and 1.73 in. into plates