IGEM:Brown/2007/Lab Protocols/Autoclave Glassware

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Autoclave Glassware

for sterilizing glassware -put in autoclavable tub -open autoclave door, put in and tighten door -set time to 20 mins -set mode knob to dry exhaust -turn handle to sterilize -come back in 45 mins and make sure everything is dry

for autoclaving LB -put in auto clavable tub with water in teh bottom -set time to 20 mins -set mode to slow exhaust -turn knob to sterilize -make sure time light comes on -come back in 20 mins and take out flask