IGEM:Berkeley/2010: Difference between revisions
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[[Template:SBB-Protocols_Micro3 | Miniprep purification of DNA]]<br> | [[Template:SBB-Protocols_Micro3 | Miniprep purification of DNA]]<br> | ||
[[Template:SBB-Protocols_Micro4 | Macherey-Nagel Miniprep]]<br> | [[Template:SBB-Protocols_Micro4 | Macherey-Nagel Miniprep]]<br> | ||
[[Template:8-Strip Machery Nagel Miniprep | 8-Strip Machery Nagel Miniprep]] <br> | |||
[[Genomic Miniprep]]<br> | [[Genomic Miniprep]]<br> | ||
[[-80 Glycerol Stocks]]<br> | [[-80 Glycerol Stocks]]<br> |
Revision as of 15:28, 14 July 2010
Project Name
Project Summary The Berkeley iGEM 2010 team is trying to apply synthetic biology to this problem. We are engineering bacteria that can deliver DNA into the choano. Choanos are predatory, which makes our job a bit simpler. Once our bacteria is engulfed by the choano, it will be programmed to burst using a self-lysis device. Then, proteins we have placed inside the bacteria will go into action. First, we have designed a vacuole-buster device that will burst the small food membrane inside the choano that held the bacteria, spewing the contents into the cytoplasm of the cell. In the cytoplasm, a transposon/transposase device tagged with a nuclear localization device will move to the nucleus. In the nucleus, the transposase will splice the transposon into the choanoflagellate DNA. |