IGEM:Berkeley/2010: Difference between revisions

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'''Addressable Cell-to-Cell Communication'''


*[[Media:071906-JCAGroupMeeting.ppt| 071906 JCA Group Meeting]]
*[[Media:060506-Meeting.ppt| 060506 Meeting]]
*[[Media:Berkeley 2005 Jamboree.ppt| Berkeley 2005 'Jamboree']]
*[http://parts2.mit.edu/wiki/index.php/UC_Berkeley_2005 2005 Project]
*[http://parts.mit.edu/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2006&group=iGEM2006_Berkeley '''2006 Parts List''']
'''Useful Links'''
'''Useful Links'''
*[[IGEM|iGEM]]
*[http://parts2.mit.edu/wiki/index.php/University_of_California_Berkeley_2006 UC Berkeley iGEM wiki]
*[http://parts2.mit.edu/wiki/index.php/Main_Page iGEM wiki]
*[http://parts.mit.edu/registry/index.php/Main_Page Registry of Standard Biological Parts]
*[http://parts2.mit.edu/r/parts/partsdb/pgroup.cgi?pgroup=iGEM&group=iGEM_Berkeley 2005 Biobricks]
*[[Image:iGEM2006Distribution.xls|iGEM2006 Distribution compiled list]]
*[http://parts2.mit.edu/wiki/index.php/University_of_California_Berkeley_2006 | iGEM site]





Revision as of 20:39, 6 June 2010


Project Name

Project Summary


<html> <img src="http://openwetware.org/images/b/b9/Icon_board.png" alt="Resources"> </html>Team


Undergrads
Amy Kristofferson
Conor McClune
Tahoura
Daniela
Christoph Neyer

Graduate Students
Tim Hsiau

Postdocs

Insert names here

Advisors
Chris Anderson
Terry Johnson

<html> <img src="http://openwetware.org/images/e/e2/Icon_info.png" alt="News" border="0"> </html>Tools


Lab calendar

Lab Notebooks

Amy
Conor
Daniela
Tahoura
Christoph
Tim
The Details

Oligonucleotides
Gel Pictures
Sequencing
Construction Files
-80 Stocks


Procedures

Polymerase Reactions
Wobble Reaction
Cloning by PCR
SOEing PCR
PCA Gene Synthesis
QuikChange

Cleanup Reactions
Regular Zymo Cleanup
Small-Frag Zymo Cleanup
Zymo Gel Purification

Digestions Reactions
EcoRI/BamHI Digest of Wobble Products
EcoRI/BamHI Digest of PCR Products
BglII Digest of EIPCR Products
Ligation of EcoRI/BamHI digests
Ligation of EIPCR digests
Analytical digests (Mapping)

Bacteria Manipulations
Transformation by heat-shock
Picking of colonies
Miniprep purification of DNA
Macherey-Nagel Miniprep

Other
LR Gateway Transfers
EcoRI/BamHI Part Transfers
What do I do with my sequencing data?
Small Scale Competent Cell Transformation
ELISA


Subgroup Notebooks



<html> <img src="http://openwetware.org/images/b/b9/Icon_board.png" alt="Resources"> </html>Project


Useful Links


Subgroup Strategies, Overview