Difference between revisions of "IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/08"

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==Entry title==
 
==Entry title==
 
* Digest more LacI on amp backbone with S and P. Digest more RBS-TesA with X and P.
 
* Digest more LacI on amp backbone with S and P. Digest more RBS-TesA with X and P.
: TesA+RBS insert with LacI+backbone
+
 
 
{| {{table}} border="1" align=center <!-- Digestion table -->
 
{| {{table}} border="1" align=center <!-- Digestion table -->
 
|- valign="top"
 
|- valign="top"

Revision as of 10:40, 8 August 2014

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Entry title

  • Digest more LacI on amp backbone with S and P. Digest more RBS-TesA with X and P.
  • Clean and concentrate LacI
  • Run RBS-TesA on a 1% agarose gel. Gel extract on Monday.
Reagent RBS-TesA (μL) LacI (μL)
DNA 15.0 15.0
10X fast Digest Buffer 2.0 2.0
PstI 1.0 1.0
XbaI 1.0 .0
SpeI 0.0 1.0
H2O 1.0 1.0
37°C for 1 hour