IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/08: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==Entry title==
==Entry title==
* Digest more LacI on amp backbone with S and P. Digest more RBS-TesA with X and P.
* Digest more LacI on amp backbone with S and P. Digest more RBS-TesA with X and P.
{| {{table}} border="1" align=center <!-- Digestion table -->
|- valign="top"
| bgcolor=#cfcfcf | Reagent
| bgcolor=#cfcfcf | RBS-TesA (μL)
| bgcolor=#cfcfcf | LacI (μL)
|-
|| DNA || 15.0|| 15.0
|-
|| 10X fast Digest Buffer|| 2.0  || 2.0
|-
|| PstI || 1.0  || 1.0
|-
|| XbaI|| 1.0 || .0
|-
|| SpeI || 0.0 || 1.0
|-
|| H<sub>2</sub>O || 1.0 || 1.0
|-
|| 37°C for 1 hour
|-
* Clean and concentrate LacI
* Clean and concentrate LacI
* Run RBS-TesA on a 1% agarose gel. Gel extract on Monday.
* Run RBS-TesA on a 1% agarose gel. Gel extract on Monday.

Latest revision as of 00:10, 27 September 2017

Project name Main project page
Previous entry      Next entry

Entry title

  • Digest more LacI on amp backbone with S and P. Digest more RBS-TesA with X and P.
  • Clean and concentrate LacI
  • Run RBS-TesA on a 1% agarose gel. Gel extract on Monday.
Reagent RBS-TesA (μL) LacI (μL)
DNA 15.0 15.0
10X fast Digest Buffer 2.0 2.0
PstI 1.0 1.0
XbaI 1.0 .0
SpeI 0.0 1.0
H2O 1.0 1.0
37°C for 1 hour
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