IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/06

From OpenWetWare
Revision as of 19:03, 6 August 2014 by David P Reynolds (talk | contribs) (Entry title)
Jump to: navigation, search
Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Entry title

  • Checked plates from 8/5
  • Only + control on chloro and RBS-TesA on cholro had growth.
  • Obtained acc plasmid (chloro resistance). Started plate and liquid colonies.

ETHANOL:

  • At 8:30 AM, O.D. of cells was approx. 1.2
    • Resuspended in 30 ml PBS, adjusted O.D. to match at 1.0, resuspended in PBS again
    • Began resting cell assay (9:15 AM):
-Flask 1: 1 g/L pyruvate (.03 g)
-Flask 2: 3 g/L pyruvate (.09 g)+ 1 g/L glucose (.03 g)
-Flask 3: 3 g/L pyruvate (.09 g)(no glucose)
    • Flasks were kept in 32°C shaker/incubator with 1 ml samples taken for Hrs. 1,2,3,6, and 8. (24 Hr. sample will be taken tomorrow)