IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/05: Difference between revisions
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* Did transformation of the ligations from yesterday of LacI with RBS-Tes with both negative and positive control. | * Did transformation of the ligations from yesterday of LacI with RBS-Tes with both negative and positive control. | ||
* Started seeds | ETHANOL: | ||
*Started shake flasks of seeds started yesterday. | |||
**Took 500 μL cells, added to new 50 ml LB+ 50 μL chloramphenicol | |||
**Waited till O.D. reached .7, added 25 μL IPTG (.5 mM) (about 2:00 PM) | |||
**Let grow overnight | |||
Revision as of 19:49, 6 August 2014
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