Difference between revisions of "IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/05"

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(Entry title)
(7/5)
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==7/5 ==
 
==7/5 ==
 
* Did transformation of the ligations from yesterday of LacI with RBS-Tes with both negative and positive control.
 
* Did transformation of the ligations from yesterday of LacI with RBS-Tes with both negative and positive control.
* Started seeds of ethanol
+
ETHANOL:
 +
*Started shake flasks of seeds started yesterday.
 +
**Took 500 μL cells, added to new 50 ml LB+ 50 μL chloramphenicol
 +
**Waited till O.D. reached .7, added 25 μL IPTG (.5 mM) (about 2:00 PM)
 +
**Let grow overnight
  
  

Revision as of 18:49, 6 August 2014

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7/5

  • Did transformation of the ligations from yesterday of LacI with RBS-Tes with both negative and positive control.

ETHANOL:

  • Started shake flasks of seeds started yesterday.
    • Took 500 μL cells, added to new 50 ml LB+ 50 μL chloramphenicol
    • Waited till O.D. reached .7, added 25 μL IPTG (.5 mM) (about 2:00 PM)
    • Let grow overnight