IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/30

From OpenWetWare
Revision as of 14:04, 1 August 2014 by Scott Ashmore (talk | contribs) (Entry title)
Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Wednesday 7/30

  • Mini Prep:
Second attempt
Isolated stocks of TesA+RBS and LacI plasmids
  • Gel Extraction:
TesA+RBS cut at X and P sites
LacI cut at S and P sites
260/280 ratio for LacI is shady, ~1.002
  • Ligation:
TesA+RBS insert with LacI+backbone