Difference between revisions of "IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/30"

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(Entry title)
(Wednesday 7/30)
Line 18: Line 18:
 
* Ligation:
 
* Ligation:
 
: TesA+RBS insert with LacI+backbone
 
: TesA+RBS insert with LacI+backbone
:
+
{| {{table}} border="1" align=center <!-- Digestion table -->
 +
|- valign="top"
 +
| bgcolor=#cfcfcf | Reagent
 +
| bgcolor=#cfcfcf | 1 (μL)
 +
| bgcolor=#cfcfcf | 2 (μL)
 +
| bgcolor=#cfcfcf | Control (μL)
 +
|-
 +
|| TesA+RBS || 2.0 || 2.0 || 0.0
 +
|-
 +
|| LacI || 3.0 || 4.0 || 3.0
 +
|-
 +
|| Ligation Buffer || 1.0 || 1.0 || 1.0
 +
|-
 +
|| Ligase || 1.0 || 1.0 || 1.0
 +
|-
 +
|| H<sub>2</sub>O || 3.0 || 2.0 || 5.0
 +
|-
  
 
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Revision as of 13:12, 1 August 2014

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Wednesday 7/30

  • Mini Prep:
Second attempt
Isolated stocks of TesA+RBS and LacI plasmids
  • Gel Extraction:
TesA+RBS cut at X and P sites
LacI cut at S and P sites
260/280 ratio for LacI is shady, ~1.002
  • Ligation:
TesA+RBS insert with LacI+backbone
Reagent 1 (μL) 2 (μL) Control (μL)
TesA+RBS 2.0 2.0 0.0
LacI 3.0 4.0 3.0
Ligation Buffer 1.0 1.0 1.0
Ligase 1.0 1.0 1.0
H2O 3.0 2.0 5.0