Difference between revisions of "IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/30"

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(Autocreate 2014/07/30 Entry for IGEM:Arizona_State/2014/Notebook/asu_igem_2014)
 
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==Entry title==
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==Wednesday 7/30==
* Insert content here...
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* Mini Prep:
 +
: Second attempt
 +
: Isolated stocks of TesA+RBS and LacI plasmids
  
 +
* Gel Extraction:
 +
: TesA+RBS cut at X and P sites
 +
: LacI cut at S and P sites
 +
: 260/280 ratio for LacI is shady, ~1.002
 +
 +
* Ligation:
 +
: TesA+RBS insert with LacI+backbone
 +
:
  
 
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Revision as of 14:04, 1 August 2014

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Wednesday 7/30

  • Mini Prep:
Second attempt
Isolated stocks of TesA+RBS and LacI plasmids
  • Gel Extraction:
TesA+RBS cut at X and P sites
LacI cut at S and P sites
260/280 ratio for LacI is shady, ~1.002
  • Ligation:
TesA+RBS insert with LacI+backbone