Difference between revisions of "IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/14"

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* Ran Digestion of TesA and RBS
 
* Ran Digestion of TesA and RBS
 
TesA cut at X, P and RBS cut at S, P.  
 
TesA cut at X, P and RBS cut at S, P.  
Note: in order to ensure the viability of our PstI enzyme, we ran a control with some PstI from Haynes lab. Results are shown below. the PstI of Haynes lab had full digestion, while the iGEM PstI had a small amount of partial digestion, as made manifest by the three bands of the TesA in lane 2 of the gel.[[14_gel_.jpg]]
+
Note: in order to ensure the viability of our PstI enzyme, we ran a control with some PstI from Haynes lab. Results are shown below. the PstI of Haynes lab had full digestion, while the iGEM PstI had a small amount of partial digestion, as made manifest by the three bands of the TesA in lane 2 of the gel.[[image:14_gel_.jpg]]
  
  

Revision as of 09:19, 15 July 2014

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Entry title

  • Made LB agar plates

1 L of distilled water, 35 grams of LB agar powder. Autoclave for 30 minutes.

  • Ran Digestion of TesA and RBS

TesA cut at X, P and RBS cut at S, P. Note: in order to ensure the viability of our PstI enzyme, we ran a control with some PstI from Haynes lab. Results are shown below. the PstI of Haynes lab had full digestion, while the iGEM PstI had a small amount of partial digestion, as made manifest by the three bands of the TesA in lane 2 of the gel.14 gel .jpg