IGEM:American University/2009/Notebook/Advanced Experimental Chemistry: Fun Times in Beeghly/2013/04/03: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(Autocreate 2013/04/03 Entry for IGEM:American_University/2009/Notebook/Advanced_Experimental_Chemistry:_Fun_Times_in_Beeghly) |
|||
Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
==Welcome to Advanced Experimental Chemistry== | ==Welcome to Advanced Experimental Chemistry== | ||
to | |||
=Today's Procedure= | |||
Reaction the Hydrogels with Fluorescent Dye: | |||
#dye in dmso, add a little and mix with film in water | |||
#Let dye, ascorbic, cuso4 and hydrogel solution over night to react with dye | |||
#Cu(I) is unstable so add CuSO4 (Cu(II)) and ascorbic acid | |||
# after 24 hours take a uv-vis of the hydrogel soaked in a known volume of dH20 | |||
'''Dye Calculatations''' | |||
* 17.24µL butyne-1-ol in the hydrogel | |||
* (17.24/70.09)*.926= 2.08E-4 moles Butyne-1-ol | |||
*rxn is 1:1 however we want 1.5 * excess of dye | |||
*Dye= azide-flour 488: mw 563.35g/mol | |||
* 563.25g/mol * 2.28E-4moes = 1.28g * 1.5 =0.193 g | |||
**not enough dye so we cut off a portion of the hydrogel to use and dye | |||
'''Mass of cut hydrogel''' | |||
* mass of whole= 12.1419 g | |||
* mass of cut= 0.8810 | |||
* percent of whole = 7.26% | |||
*Original amount of dye needed= 0.014g | |||
** however, we only have 0.005 g | |||
'''Copper sulfat concentration''' | |||
*249.8*0.001moles/l*0.01l= 0.0025 g of copper sulfate | |||
*MADE A STOCK SOLUTION OF .025g/ml so 100µl =0.0025g of copper sulfate | |||
'''L-Ascorbic acid''' | |||
* 176.13g/mol * .1mol/L * 0.0L= 0.1761g | |||
*made stock solution of 1.761g per 10ml so 1 ml = .1761g | |||
'''range of dye volumes tested since did not have enough made a stock solution of 5 mg of dye in 1 ml of dmso''' | |||
*ranges: 500, 200, 100, 10 and 1 µL of dye | |||
* Added to hydrogels 9 ml of water + dye + 100µL of copper sulfate + 1 ml of ascorbic acid | |||
Revision as of 08:24, 3 April 2013
iGEM Project name 1 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Welcome to Advanced Experimental ChemistryToday's ProcedureReaction the Hydrogels with Fluorescent Dye:
Dye Calculatations
Mass of cut hydrogel
Copper sulfat concentration
L-Ascorbic acid
range of dye volumes tested since did not have enough made a stock solution of 5 mg of dye in 1 ml of dmso
|