IGEM:American University/2009/Notebook/Advanced Experimental Chemistry: Fun Times in Beeghly/2013/02/01: Difference between revisions
(Autocreate 2013/02/01 Entry for IGEM:American_University/2009/Notebook/Advanced_Experimental_Chemistry:_Fun_Times_in_Beeghly) |
|||
| Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
==Welcome to Advanced Experimental Chemistry== | ==Welcome to Advanced Experimental Chemistry== | ||
to | |||
=Today's Procedure= | |||
7 films were made using 0.5g PVOH (MW: 146K-186K) dissolve in 6+ mL water (more water was added as it became apparent more water was needed). Usual glutaraldehyde procedures were used. | |||
1) PVOH and water | |||
2) PVOH, 1mL glutaraldehyde | |||
3) PVOH, 1mL glutaraldehyde, 0.069g Isopropanol | |||
4) PVOH, 1mL glutaraldehyde, 0.0069g isopropanol | |||
5) PVOH, 1mL glutaraldehyde, 0.00069g isopropanol | |||
6) PVOH, 1mL glutaraldehyde, 0.000069g isopropanol | |||
7) PVOH and water in a small dish | |||
Isopropanol was measured using a serial dilution of pure isopropanol, 10% isopropanol, 1%isopropanol, and 0.1% isopropanol. | |||
This was calculated by taking 10 mL pure isopropanol in a falcon tube. Diluting 1mL of the isopropanol with 9mL water in another tube (10%). Diluting 1mL of that with 9mL of water (1%). And finally diluting 1mL of the 1% isopropanol with 9mL water (0.1%). Thus 0.087mL of each successive dilution was added to PVOH films 3-7. | |||
Revision as of 12:41, 1 February 2013
 iGEM Project name 1
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Welcome to Advanced Experimental ChemistryToday's Procedure7 films were made using 0.5g PVOH (MW: 146K-186K) dissolve in 6+ mL water (more water was added as it became apparent more water was needed). Usual glutaraldehyde procedures were used. 1) PVOH and water 2) PVOH, 1mL glutaraldehyde 3) PVOH, 1mL glutaraldehyde, 0.069g Isopropanol 4) PVOH, 1mL glutaraldehyde, 0.0069g isopropanol 5) PVOH, 1mL glutaraldehyde, 0.00069g isopropanol 6) PVOH, 1mL glutaraldehyde, 0.000069g isopropanol 7) PVOH and water in a small dish Isopropanol was measured using a serial dilution of pure isopropanol, 10% isopropanol, 1%isopropanol, and 0.1% isopropanol. This was calculated by taking 10 mL pure isopropanol in a falcon tube. Diluting 1mL of the isopropanol with 9mL water in another tube (10%). Diluting 1mL of that with 9mL of water (1%). And finally diluting 1mL of the 1% isopropanol with 9mL water (0.1%). Thus 0.087mL of each successive dilution was added to PVOH films 3-7.
| |
